AMFANI DA NUFIN
Matakin Karfi^®Na'urar Gwajin gaggawa ta Adenovirus (Feces) wani saurin gani neImmunoassay don gano ƙimar ƙimar adenovirus a cikin ɗan adamsamfurori na fecal.An yi nufin wannan kit ɗin don amfani a matsayin taimako a cikin ganewar asali na adenovirus
kamuwa da cuta.

GABATARWA
Enteric adenoviruses, da farko Ad40 da Ad41, sune babban dalilin gudawaa yawancin yara masu fama da cutar zawo, na biyukawai ga rotaviruses.Cutar gudawa mai saurin kisa ita ce babbar sanadin mutuwaa cikin yara ƙanana a duniya, musamman a ƙasashe masu tasowa.AdenovirusAn ware ƙwayoyin cuta a duk faɗin duniya, kuma suna iya haifar da gudawaa cikin yara duk shekara.An fi ganin cututtuka a cikin yara da ba su kai bashekaru biyu, amma an same su a cikin marasa lafiya na kowane zamani.Nazarin ya nuna cewa adenoviruses suna hade da 4-15% na dukakamuwa da cutar gastroenteritis a asibiti.

Gastroenteritis mai alaka da adenovirus yana taimakawaa cikin kafa etiology na gastroenteritis da kuma kula da haƙuri masu dangantaka.Wasu fasahohin bincike kamar su microscope na lantarki (EM) danucleic acid hybridization yana da tsada kuma yana da ƙarfin aiki.An ba dayanayin iyakance kansa na kamuwa da cutar adenovirus, irin wannan tsada dagwaje-gwajen aiki mai ƙarfi bazai zama dole ba.

KA'IDA
Na'urar gwajin gaggawa ta Adenovirus (Feces) tana gano adenovirusta hanyar fassarar gani na ci gaban launi akan cikitsiri.Anti-adenovirus antibodies suna dawwama a kan yankin gwaji namembrane.A lokacin gwaji, samfurin yana amsawa da ƙwayoyin rigakafin adenovirusconjugated zuwa launi barbashi da precoated uwa samfurin kushin na gwajin.Cakuda daga nan yana ƙaura ta cikin membrane ta aikin capillary kuma yana hulɗatare da reagents a kan membrane.Idan akwai isasshen adenovirus a cikin samfurin, aband mai launi za ta kasance a yankin gwaji na membrane.Kasancewar wannanband mai launi yana nuna sakamako mai kyau, yayin da rashinsa yana nuna mummunansakamako.Bayyanar band mai launi a yankin sarrafawa yana aiki azaman akula da tsari, yana nuna cewa ingancin samfurin ya kasancekara da kuma membrane wicking ya faru.

TSARI
Kawo gwaje-gwaje, samfurori, buffer da/ko sarrafawa zuwa zafin jiki(15-30 ° C) kafin amfani.
1. Tarin samfurori da riga-kafi:
1) Yi amfani da busassun kwantena masu tsabta don tarin samfurori.Mafi kyawun sakamako zai kasancesamu idan an yi gwajin a cikin sa'o'i 6 bayan tattarawa.
2) Don ƙwaƙƙwaran samfurori: Cire kuma cire abin shafa bututun dilution.KasanceYi hankali kada a zubar ko zubar da bayani daga bututu.Tattara samfurorita hanyar shigar da sandar applicator a cikin aƙalla shafuka 3 daban-daban nanajasa don tattara kusan 50 MG na najasa (daidai da 1/4 na fis).Don samfurori na ruwa: Rike pipette a tsaye, fecalsamfurori, sa'an nan kuma canja wurin digo 2 (kimanin 80 µL) zuwa cikinbututun tarin samfuri mai ɗauke da buffer cirewa.
3) Mayar da applicator baya cikin bututu da dunƙule hula sosai.KasanceYi hankali kada a karya tip na dilution tube.
4) Girgiza bututun samfurin da ƙarfi don haɗa samfurin dada hakar buffer.Samfuran da aka shirya a cikin bututun tarin samfurinAna iya adana shi na tsawon watanni 6 a -20 ° C idan ba a gwada shi ba a cikin awa 1 bayanshiri.

2. Gwaji
1) Cire gwajin daga jakar da aka hatimi, sannan a sanya shimai tsabta, matakin saman.Yi wa gwajin lakabi da majiyyaci ko sarrafawaganewa.Don sakamako mafi kyau, yakamata a yi gwajin a cikin guda ɗayaawa.
2) Yin amfani da takarda mai laushi, karya ƙarshen bututun dilution.Rikebututun a tsaye kuma a ba da digo 3 na bayani a cikin samfurin da kyau(S) na na'urar gwaji.Ka guje wa tarko kumfa a cikin samfurin rijiyar (S), kuma kar a ƙara
duk wani bayani ga taga sakamako.Yayin da gwajin ya fara aiki, launi zai yi ƙaura a cikin membrane.

3. Jira band(s) masu launin su bayyana.Ya kamata a karanta sakamakon a 10mintuna.Kar a fassara sakamakon bayan mintuna 20.

Lura:Idan samfurin bai yi ƙaura ba saboda kasancewar barbashi, centrifugesamfuran da aka fitar da ke ƙunshe a cikin buffer buffer.Tara 100µL namai girma, saka cikin samfurin rijiyar (S) na sabon na'urar gwaji kuma sake farawa, bin umarnin da aka kwatanta a sama.

Takaddun shaida