Novel Coronavirus (SARS-CoV-2) Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Kayan Gida

Short Bayani:

Labarin kwaroronavirus shine kwayar RNA, wacce ta kunshi sunadarai da nucleic acid. Kwayar cutar ta mamaye jikin mai gida (ɗan adam), ta shiga cikin ƙwayoyin cuta ta hanyar karɓar mai karɓar mai karɓa ta ACE2, kuma ta kwafa a cikin ƙwayoyin halittar, wanda ke haifar da tsarin garkuwar jiki na mutum don amsawa ga maharan ƙasashen waje da kuma kera wasu ƙwayoyin cuta. Sabili da haka, zazzabin nucleic acid da antigens, da takamaiman abubuwan rigakafi game da littafin coronavirus ana iya amfani dasu bisa ka'ida azaman takamaiman masanan kimiyyar halittu don gano littafin coronavirus. Don gano acidic na nucleic, ana amfani da fasahar RT-PCR sosai.

Novel Coronavirus (SARS-CoV-2) Multiplex Real-Time PCR Kit an yi amfani da shi don cimma nasarar gano SARS_CoV-2 kwayar RNA da aka ciro daga nasopharyngeal swabs, oropharyngeal swabs, sputum da BALF daga marasa lafiya a haɗuwa da FDA / CE Tsarin cirewa na IVD da kuma dandamali na PCR da aka zayyana a sama.

An shirya kit ɗin don yin amfani da shi ta ƙwararrun ma'aikata da aka horar


Bayanin Samfura

Alamar samfur

Wannan kayan kwalliyar PCR mai matukar mahimmanci, ana samunsu a cikin siyollized (tsarin daskarewar daskarewa) don ajiyar lokaci mai tsawo. Za'a iya jigilar kit ɗin kuma a adana ta a zafin ɗaki kuma ta daidaita shekara ɗaya. Kowane bututu na premix ya ƙunshi dukkan abubuwan reagents da ake buƙata don haɓaka PCR, gami da Reverse-transcriptase, Taq polymerase, primers, probes, da dNTPs substrates. Abin sani kawai yana buƙatar ƙara ruwa mai 13ul da 5ul wanda aka cire samfurin RNA, sa'annan ana iya gudanar dashi da haɓaka akan kayan aikin PCR.

Injin qPCR ya kamata ya cika abubuwan da ake buƙata:
1. Fit 8 tsiri PCR bututu mai girma 0.2 ml
2. Yi sama da tashoshin ganowa huɗu:

Channel

Jin daɗi (nm)

Fitar (nm)

Pre-Calibrated Dyes

1.

470

525

FAM, SYBR Kore Na

2

523

564

VIC, HEX, TET, JOE

3.

571

621

ROX, TEXAS-RED

4

630

670

CY5

Tsarin PCR:
7500Real-Time PCR System, Biorad CF96, iCycler iQ ™ Real-Time PCR Detection System, Stratagene Mx3000P, Mx3005P

Halin wahalar jigilar kayan sanyi na Novel Coronavirus mai gano kwayar cutar nukiliya
Lokacin da aka kwashe reagent na gano nucleic acid a nesa mai nisa, ana buƙatar (-20 ± 5) chain ajiyayyen sarkar sanyi da sufuri don tabbatar da haɓakar enzyme a cikin reagents ya kasance mai aiki. Don tabbatar da cewa zafin ya kai mizanin, ana buƙatar kilogram da yawa na ƙanƙarar ƙanƙara ga kowane akwati na gwajin kwayar nucleic har ma da ƙasa da 50g, amma zai iya wuce kwana biyu ko uku. Dangane da aikin masana'antu, ainihin nauyin reagents da masana'antun ke bayarwa ƙasa da 10% (ko kuma ƙasa da wannan ƙimar) na akwatin. Yawancin nauyin ya fito ne daga busassun kankara, kayan kankara da akwatunan kumfa, don haka farashin sufuri yana da matuƙar tsayi.

A watan Maris na 2020, COVID-19 ya fara ɓarkewa a cikin babban sihiri a ƙasashen waje, kuma buƙatar Noagar Coronavirus reagent na gano nucleic acid ya karu sosai. Duk da tsadar fitar da reagents a cikin sarkar sanyi, yawancin masana'antun na iya karɓar shi saboda yawa da riba mai yawa.

Koyaya, tare da inganta manufofin fitarwa na ƙasa don kayayyakin yaƙi da annoba, gami da haɓaka ikon ƙasa game da kwararar mutane da kayan aiki, akwai tsawaitawa da rashin tabbas a lokacin jigilar kayayyaki na reagents, wanda ya haifar da fitattun matsalolin samfura ta hanyar sufuri. Tsawon lokacin sufuri (lokacin safarar kusan rabin wata yana da yawa) yana haifar da lalacewar samfuran da yawa lokacin da samfurin ya isa ga abokin harka. Wannan ya dame yawancin masana'antun acid nucleic acid.

Lyophilized technology for PCR reagent ya taimaka safarar Novel Coronavirus nucleic acid gano reagent duniya

Za'a iya jigilar abubuwan reagent na PCR da ke cikin liophilized kuma a adana su a cikin zafin jiki na ɗaki, wanda hakan ba zai iya rage farashin sufuri ba kawai, amma kuma ya guji matsalolin inganci da tsarin sufuri ya haifar. Sabili da haka, sake fasalin reagent shine hanya mafi kyau don magance matsalar safarar fitarwa.

Lyophilization ya haɗa da daskarewa da mafita cikin yanayi mai ƙarfi, sa'annan kuma sublimate da raba tururin ruwa a ƙarƙashin yanayi mara kyau. Maganin busassun ya rage a cikin akwati tare da abin da ke ciki da aiki iri ɗaya. Idan aka kwatanta da magungunan ruwa na yau da kullun, abun da ke tattare da Novel Coronavirus mai gano sinadarin nucleic acid wanda Liming Bio ya samar yana da halaye masu zuwa:

Stabilityarfin ƙarfin zafi mai ƙarfi: ana iya yin shi tare da jiyya a 56 ℃ na tsawon kwanaki 60, kuma ilimin halittar jiki da aikin reagent sun canza.
Al'ada ajiyar zafin jiki da sufuri: babu buƙatar sarkar sanyi, babu buƙatar adanawa a ƙarancin zafin jiki kafin buɗewa, saki cikakken filin ajiyar sanyi.
Shirya don amfani: lyophilizing na dukkan abubuwan da aka gyara, babu buƙatar tsarin daidaitawa, guje wa asarar abubuwan haɗi tare da babban ɗanko kamar enzyme.
Mahara da yawa a cikin bututu ɗaya: makasudin ganowa ya rufe sabon kwayar coronavirus ORF1ab gene, N gene, S gene don gujewa kwayar cutar genovariation. Don rage mummunan ƙaryar, ana amfani da kwayar RNase P ta ɗan adam azaman sarrafa ciki, don saduwa da buƙatun asibiti don samfurin ƙirar inganci.


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