AMFANI DA NUFIN
Matakin Karfi^®Gwajin Procalcitonin shine saurin rigakafi-chromatographickididdigewa don gano rabin ƙididdiga na Procalcitonin a cikin ƙwayar ɗan adam koplasma.Ana amfani dashi don tantancewa da sarrafa maganin mai tsanani,kamuwa da cuta na kwayan cuta da sepsis.

GABATARWA
Procalcitonin (PCT) ƙaramin furotin ne wanda ya ƙunshi ragowar amino acid 116tare da nauyin kwayoyin halitta kusan 13 kDa wanda aka fara kwatantada Moullec et al.a shekarar 1984.Ana samar da PCT kullum a cikin ƙwayoyin C na thyroid gland.A shekarar 1993, dagirman matakin PCT a cikin marasa lafiya tare da tsarin kamuwa da cuta na asalin ƙwayoyin cutaAn ba da rahoto kuma yanzu ana ɗaukar PCT a matsayin babban alamar cutatare da kumburi tsari da sepsis.Ƙimar bincike naPCT yana da mahimmanci saboda kusancin kusanci tsakanin tattarawar PCT datsananin kumburi.An nuna cewa "mai kumburi" PCT basamar a cikin C-cells.Kwayoyin asalin neuroendocrine tabbas sune tushenna PCT a lokacin kumburi.

KA'IDA
Matakin Karfi^®Gwajin gaggawa na Procalcitonin yana gano Procalcitonin ta hanyar ganifassarar ci gaban launi a kan tsiri na ciki.Procalcitoninmonoclonal antibody ba shi da motsi a yankin gwaji na membrane.Lokacingwaji, samfurin yana amsawa tare da ƙwayoyin rigakafi na anti-Procalcitonin monoclonalconjugated zuwa ga barbashi masu launi kuma an riga an rufe su a kan kushin haɗin gwiwa na gwajin.A cakuda sa'an nan ƙaura ta cikin membrane ta capillary mataki da kumaYana hulɗa tare da reagents akan membrane.Idan akwai isassun Procalcitonin a cikisamfurin, band mai launi zai samar a yankin gwaji na membrane.Thekasancewar wannan band mai launi yana nuna sakamako mai kyau, yayin da babu shiyana nuna sakamako mara kyau.Bayyanar band mai launi a sarrafawayankin hidima a matsayin tsari iko, nuna cewa dace girma naan ƙara samfurin kuma an sami ɓacin rai.Haɓaka launi daban-daban a cikin yankin layin gwaji (T) yana nuna sakamako mai kyauyayin da adadin Procalcitonin za a iya ƙididdige shi da ƙima ta hanyarkwatankwacin ƙarfin layin gwajin zuwa ƙimar layin tunani akankatin fassarar.Rashin layin launi a yankin layin gwaji(T)yana nuna sakamako mara kyau.

MATAKAN KARIYA
Wannan kit ɗin don amfanin bincike ne kawai na IN VITRO.
n Karanta umarnin a hankali kafin yin gwajin.
∎ Wannan samfurin ba ya ƙunsar kowane kayan tushen ɗan adam.
∎ Kar a yi amfani da abun ciki na kit bayan ranar karewa.
∎ Karɓar duk samfuran a matsayin masu iya kamuwa da cuta.
∎ Bi daidaitaccen tsarin Lab da ka'idojin kare lafiyar halittu don kulawa dazubar da abu mai yuwuwar kamuwa da cuta.Lokacin da tsarin tantancewa ya kasancecikakke, zubar da samfurori bayan autoclaving su a 121 ℃ don akalla20 min.A madadin, ana iya bi da su tare da 0.5% sodium Hypochloritena sa'o'i kafin a zubar.
∎ Kada a yi amfani da pipette ta baki kuma kada a shan taba ko ci yayin da ake tantancewa.
■ Sanya safar hannu yayin duk aikin.