Gwajin Fibronectin na Fetal
AMFANI DA NUFI
Matakin Karfi®Gwajin PROM gwaji ne na immunochromatographic da aka fassara ta gani da nufin yin amfani da shi don gano ƙimar fibronectin tayi a cikin ɓoyewar mahaifa.Kasancewar fibronectin tayi a cikin ɓoye na cervicovaginal tsakanin makonni 22, kwanaki 0 da makonni 34, kwanakin 6 na ciki shinehade da haɓakar haɗarin haihuwa kafin haihuwa.
GABATARWA
Bayarwa da wuri, wanda Cibiyar Nazarin Magunguna ta Amirka ta ayyana a matsayin bayarwa kafin mako na 37 na ciki, yana da alhakin yawancin cututtuka da mace-mace marasa chromosomal.Alamomin barazanar haihuwa kafin haihuwa sun hada da ciwon mahaifa, canjin zubar da jini, zubar jinin al'ada, ciwon baya, rashin jin dadi na ciki, matsawar pelvic, da kuma kumbura.Hanyoyin bincike don gano barazanar haihuwa kafin haihuwa sun haɗa da sa ido kan ayyukan mahaifa da aikin gwajin ƙwayar mahaifa na dijital, wanda ke ba da izinin kimanta girman mahaifa.An nuna waɗannan hanyoyin suna da iyakancewa, yayin da ƙananan ƙwayar mahaifa (< 3 centimeters) da kuma aikin mahaifa suna faruwa akai-akai kuma ba lallai ba ne a gano cutar da haihuwa.Yayin da aka kimanta alamomin sinadarai da yawa, babu wanda aka yarda da shi don amfanin asibiti mai amfani.
Fibronectin tayi (fFN), isoform na fibronectin, wani hadadden glycoprotein ne mai mannewa tare da nauyin kwayoyin halitta na kusan 500,000 daltons.Matsuura da abokan aiki sun bayyana wani antibody monoclonal da ake kira FDC-6, wanda ke gane musamman III-CS, yankin da ke bayyana ɓangarorin tayi na fibronectin.Immunohistochemical binciken na placentae ya nuna cewa fFN nekeɓaɓɓe ga matrix extracellular na yankin da ke bayyana mahaɗinna bangaren uwa da tayi a cikin mahaifa.
Za a iya gano fibronectin na tayi a cikin ɓoyayyen ɓoyayyen mahaifa na mata a duk lokacin da suke da juna biyu ta hanyar amfani da maganin rigakafi na monoclonal antibody.Fibronectin tayi yana haɓaka a cikin ɓoyayyen ɓoyayyen mahaifa a lokacin farkon ciki amma yana raguwa daga makonni 22 zuwa 35 a cikin al'ada.Ba a fahimci muhimmancin kasancewarsa a cikin farji a farkon makonni na ciki ba.Koyaya, yana iya zama kawai yana nuna haɓakar al'ada na yawan trophoblast masu yawa da kuma mahaifa.Gano fFN a cikin sirrin mahaifa tsakanin makonni 22, kwanaki 0 da makonni 34, an ba da rahoton cewa ciki na kwanaki 6 yana da alaƙa da haihuwa a cikin bayyanar cututtuka kuma tsakanin makonni 22, kwanaki 0 da makonni 30, kwanaki 6 a cikin mata masu ciki asymptomatic.
KA'IDA
Matakin Karfi®Gwajin fFN yana amfani da immunochromatographic launi, fasahar kwararar capillary.Tsarin gwajin yana buƙatar solubilization na fFN daga swab na farji ta hanyar haɗa swab a cikin Sample Buffer.Sa'an nan kuma za a ƙara gauraye samfurin buffer zuwa gwajin kaset da kyau kuma cakuda yana ƙaura tare da saman membrane.Idan fFN ta kasance a cikin samfurin, zai samar da hadaddun tare da farkon anti-fFN antibody hade da barbashi masu launi.Sa'an nan za a ɗaure hadaddun da wani anti-fFN na biyu wanda aka lulluɓe akan membrane na nitrocellulose.Bayyanar layin gwajin bayyane tare da layin sarrafawa zai nuna sakamako mai kyau.
KIT ABUBUWAN
| 20 Kowane mutum pakced gwajin na'urorin | Kowace na'ura tana ƙunshe da tsiri mai launin conjugates da reactive reagents waɗanda aka riga aka yi musu rufi a yankuna masu dacewa. |
| 2Ana cirewaBuffer vial | 0.1 M Phosphate buffered saline (PBS) da 0.02% sodium azide. |
| 1 swab mai inganci (kan buƙata kawai) | Ya ƙunshi fFN da sodium azide.Don sarrafawa na waje. |
| 1 swab mara kyau (kan buƙata kawai) | Ba ya ƙunshi fFN.Don sarrafa waje. |
| 20 Bututun cirewa | Don samfuran shirye-shiryen amfani. |
| 1 Wurin aiki | Wuri don riƙe buffer vials da bututu. |
| 1 Saka kunshin | Don umarnin aiki. |
KAYAN DA AKE BUKATA AMMA BA'A SAMU BA
| Mai ƙidayar lokaci | Don amfani da lokaci. |
MATAKAN KARIYA
∎ Don ƙwararrun ƙwararrun bincike na in vitro amfani kawai.
■ Kada a yi amfani bayan ranar karewa da aka nuna akan kunshin.Kar a yi amfani da gwajin idan jakar jakar ta ta lalace.Kar a sake amfani da gwaje-gwaje.
Wannan kit ɗin ya ƙunshi samfuran asalin dabba.Ingantacciyar masaniyar asalin da/ko yanayin tsaftar dabbobi baya bada garantin gabaɗayan rashi ƙwayoyin cuta masu yaduwa.Don haka, ana ba da shawarar cewa a kula da waɗannan samfuran azaman masu iya kamuwa da cuta, kuma a sarrafa su tare da kiyaye ƙa'idodin aminci na yau da kullun (kada a sha ko shaƙa).
∎ Guje wa ƙetaren samfura ta hanyar amfani da sabon akwati na tattara samfuran ga kowane samfurin da aka samu.
■ Karanta tsarin duka a hankali kafin yin kowane gwaji.
■ Kada ku ci, sha ko shan taba a wurin da ake sarrafa samfurori da kayan aiki.Yi amfani da duk samfuran kamar suna ɗauke da ƙwayoyin cuta.Kula da ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙwayoyin cuta a duk tsawon aikin kuma bi ƙa'idodin ƙa'idodin don zubar da samfuran daidai.Sanya tufafi masu kariya kamar sutturar dakin gwaje-gwaje, safar hannu da za a iya zubarwa da kariyar ido lokacin da aka tantance samfurori.
∎ Kar a yi musanya ko hada reagents daga kuri'a daban-daban.Kar a haxa iyakoki na mafita.
Danshi da zafin jiki na iya yin illa ga sakamako.
∎ Lokacin da aikin tantancewa ya ƙare, zubar da swabs a hankali bayan an haɗa su ta atomatik a 121 ° C na akalla minti 20.A madadin, ana iya bi da su tare da 0.5% sodium hypochloride (ko bleach-rike gida) na awa ɗaya kafin zubar.Ya kamata a jefar da kayan gwajin da aka yi amfani da su daidai da ƙa'idodin gida, jihohi da/ko na tarayya.
∎ Kada a yi amfani da goga na cytology tare da masu ciki.
AJIYA DA KWANTA
∎ Yakamata a adana kit ɗin a 2-30°C har sai an buga kwanan watan ƙarewa akan jakar da aka rufe.
■ Dole ne gwajin ya kasance a cikin jakar da aka rufe har sai an yi amfani da shi.
■ Kar a daskare.
∎ Yakamata a kula don kare abubuwan da ke cikin wannan kit din daga kamuwa da cuta.Kar a yi amfani da shi idan akwai shaidar gurɓataccen ƙwayar cuta ko hazo.Cututtukan halittu na kayan aikin rarrabawa, kwantena ko reagents na iya haifar da sakamako na ƙarya.
TATTAUNAWA DA ARZIKI
∎ Yi amfani da swabs na Dacron ko Rayon kawai tare da robobi.Ana ba da shawarar yin amfani da swab ɗin da masana'anta ke bayarwa (Ba a ƙunshi swabs a cikin wannan kit ɗin ba, don bayanin oda, da fatan za a tuntuɓi masana'anta ko masu rarraba gida, lambar kasida ita ce 207000).Ba a inganta swabs daga wasu masu kaya ba.Swabs tare da tukwici na auduga ko katako na katako ba a ba da shawarar ba.
∎ Ana samun sinadarai na cervicovaginal daga farji na baya na farji.Tsarin tarin ana nufin ya zama mai laushi.Tari mai ƙarfi ko ƙarfi, gama gari don al'adun ƙwayoyin cuta, ba a buƙata.Yayin jarrabawar hatsabibin, kafin kowane gwaji ko magudin mahaifar mahaifa ko farji, a hankali juya tip ɗin applicator a cikin farjin baya na farji na kusan daƙiƙa 10 don ɗaukar ɓoyewar mahaifa.Ƙoƙarin ƙoƙarce-ƙoƙarce na gaba don cika tip ɗin mai amfani na iya lalata gwajin.Cire applicator kuma yi gwajin kamar yadda aka umurce su a ƙasa.
■ Sanya swab zuwa bututun cirewa, idan ana iya yin gwajin nan da nan.Idan gwajin nan da nan ba zai yiwu ba, ya kamata a sanya samfuran marasa lafiya a cikin busassun bututun sufuri don ajiya ko jigilar kaya.Ana iya adana swabs na awanni 24 a zazzabi na ɗaki (15-30°C) ko sati 1 a 4°C ko fiye da wata 6 a -20°C.Duk samfuran yakamata a bar su su kai zafin daki na 15-30 ° C kafin gwaji.
TSARI
Kawo gwaje-gwaje, samfurori, buffer da/ko sarrafawa zuwa zafin jiki (15-30°C) kafin amfani.
■ Sanya bututu mai tsafta a wurin da aka keɓe na wurin aiki.Ƙara 1ml na Mai Buffer Extraction zuwa bututun hakar.
■ Saka swab ɗin a cikin bututu.Haxa maganin da ƙarfi ta hanyar jujjuya swab da ƙarfi a gefen bututun na tsawon aƙalla sau goma (yayin nitse).Ana samun sakamako mafi kyau lokacin da samfurin ya haɗu da ƙarfi a cikin maganin.
■ Fitar da ruwa mai yawa kamar yadda zai yiwu daga swab ta hanyar danna gefen bututun cirewa mai sassauƙa yayin da ake cire swab.Aƙalla 1/2 na maganin buffer samfurin dole ne ya kasance a cikin bututu don isassun ƙaura na capillary ya faru.Saka hular kan bututun da aka fitar.
Yi watsi da swab a cikin kwandon shara mai haɗari mai haɗari.
∎ Samfuran da aka ciro na iya ajiyewa a cikin daki na tsawon mintuna 60 ba tare da sun shafi sakamakon gwajin ba.
∎ Cire gwajin daga jakar da aka hatimi, sa'annan a sanya shi a kan tsaftataccen wuri.Yi wa na'urar lakabi da majiyyaci ko ganewar sarrafawa.Don samun sakamako mafi kyau, yakamata a yi gwajin a cikin sa'a ɗaya.
∎ Ƙara ɗigo 3 (kimanin 100 µl) na samfurin da aka ciro daga Tube Extraction zuwa samfurin rijiyar akan kaset ɗin gwaji.
Guji kama kumfa mai iska a cikin samfurin rijiyar (S), kuma kar a jefa kowane bayani a cikin taga kallo.
Yayin da gwajin ya fara aiki, za ku ga launi yana motsawa a cikin membrane.
■ Jira band(s) masu launin su bayyana.Ya kamata a karanta sakamakon a minti 5.Kar a fassara sakamakon bayan mintuna 5.
Yi watsi da bututun gwajin da aka yi amfani da su da Kaset ɗin Gwaji a cikin kwandon shara mai dacewa.
FASSARAR SAKAMAKO
| KYAUTASAKAMAKO:
| Makada masu launi biyu suna bayyana akan membrane.Ƙungiya ɗaya yana bayyana a cikin yankin sarrafawa (C) kuma wani rukunin yana bayyana a yankin gwaji (T). |
| KARANTASAKAMAKO:
| Ƙungiya mai launi ɗaya kawai ya bayyana a cikin yankin sarrafawa (C).Babu makaɗa mai launi da ya bayyana a yankin gwaji (T). |
| BA YAWASAKAMAKO:
| Ƙungiyar sarrafawa ta kasa bayyana.Sakamako daga kowane gwajin da bai samar da rukunin sarrafawa ba a ƙayyadadden lokacin karantawa dole ne a watsar da shi.Da fatan za a sake duba tsarin kuma a maimaita tare da sabon gwaji.Idan matsalar ta ci gaba, daina amfani da kit ɗin nan da nan kuma tuntuɓi mai rarrabawa na gida. |
NOTE:
1. Ƙarfin launi a yankin gwaji (T) na iya bambanta dangane da ƙaddamar da abubuwan da ake nufi da ke cikin samfurin.Amma matakin abubuwan ba za a iya ƙayyade ta wannan gwajin ingancin ba.
2. Rashin isassun ƙarar samfurin, tsarin aiki da ba daidai ba, ko yin gwaje-gwajen da suka ƙare sune mafi kusantar dalilai na gazawar bandeji.
KYAUTATA KYAUTA
∎ An haɗa tsarin sarrafawa na ciki a cikin gwajin.Ƙungiya mai launi da ke bayyana a cikin yankin sarrafawa (C) ana ɗaukarsa azaman ingantaccen tsarin kulawa na ciki.Yana tabbatar da isassun ƙarar samfurin da ingantacciyar dabarar tsari.
∎ Ana iya samar da tsarin sarrafawa na waje (kan buƙata kawai) a cikin kayan don tabbatar da cewa gwaje-gwajen suna aiki yadda ya kamata.Hakanan, ana iya amfani da Sarrafa don nuna kyakkyawan aiki ta afaretan gwaji.Don yin gwajin sarrafawa mai inganci ko mara kyau, kammala matakan da ke cikin sashin Tsarin Gwaji da ke kula da swab mai sarrafawa daidai da swab samfuri.
IYAKA NA GWAJI
1. Za a iya amfani da wannan ƙididdiga kawai don gano ƙimar fibronectin fetal a cikin ɓarna na cervicovaginal.
2. Ya kamata a yi amfani da sakamakon gwajin koyaushe tare da sauran bayanan asibiti da na dakin gwaje-gwaje don sarrafa haƙuri.
3. Ya kamata a samu samfurori kafin a yi gwajin dijital ko magudin mahaifa.Magani na cervix na iya haifar da sakamako mai kyau na ƙarya.
4. Kada a tattara samfurori idan mai haƙuri ya yi jima'i a cikin sa'o'i 24 don kawar da sakamakon karya.
5. Marasa lafiya da ake zargi ko an sani bazuwar mahaifa, placenta previa, ko matsakaici ko babban zubar jinin al'ada bai kamata a gwada ba.
6. Kada a gwada marasa lafiya tare da cerclage.
7. Halayen wasan kwaikwayo na StrongStep®Gwajin fFN ya dogara ne akan nazarin mata masu ciki.Ba a tabbatar da aikin ba a kan marasa lafiya masu ciki da yawa, misali, tagwaye.
8. Mataki mai ƙarfi®Ba a nufin gwajin fFN da za a yi ba a gaban fashewar membranes na amniotic kuma ya kamata a yanke hukunci kafin a gudanar da gwajin.
HALAYEN YI
Tebur: Gwajin StrongStep® fFN vs. Wani nau'in Gwajin fFN
| Hankalin Dangi: 97.96% (89.13% -99.95%)* Ƙimar Dangi: 98.73% (95.50% -99.85%)* Gabaɗaya Yarjejeniyar: 98.55% (95.82% -99.70%)* *95% Tsakanin Amincewa |
| Wani iri |
| ||
| + | - | Jimlar | |||
| Mataki mai ƙarfi®fFn Gwaji | + | 48 | 2 | 50 | |
| - | 1 | 156 | 157 | ||
|
| 49 | 158 | 207 | ||
Analytic hankali
Mafi ƙarancin adadin fFN a cikin samfurin da aka fitar shine 50μg/L.
Daga cikin mata masu alama, matakan haɓaka (≥ 0.050 μg/mL) (1 x 10-7 mmol/L) na fFN tsakanin makonni 24, kwanakin 0 da makonni 34, kwanakin 6 suna nuna haɗarin bayarwa a cikin ≤ 7 ko ≤ 14 kwanaki daga tarin samfurin.Daga cikin mata masu asymptomatic, haɓakar matakan fFN tsakanin makonni 22, kwanaki 0 da makonni 30, kwanaki 6 suna nuna haɗarin haihuwa a cikin ≤ 34 makonni, kwanakin 6 na ciki.An kafa yankewar 50 μg/L fFN a cikin binciken da aka gudanar da yawa don kimanta haɗin kai tsakanin maganganun fibronectin tayi a lokacin daukar ciki da haihuwa.
Abubuwa Masu Tsangwama
Dole ne a kula don kada a gurɓata mai amfani ko ɓoyewar mahaifa tare da man shafawa, sabulu, magungunan kashe kwayoyin cuta, ko mayukan shafawa.Man shafawa ko man shafawa na iya tsoma baki cikin jiki tare da sha samfurin akan na'urar.Sabulu ko maganin kashe kwayoyin cuta na iya tsoma baki tare da maganin antibody-antigen.
An gwada abubuwan da za su iya shiga tsakani a ɗimbin yawa waɗanda za a iya samun su da kyau a cikin ɓoyewar mahaifa.Abubuwan da ke gaba ba su tsoma baki a cikin binciken ba lokacin da aka gwada su a matakan da aka nuna.
| Abu | Hankali | Abu | Hankali |
| Ampicillin | 1.47 mg/ml | Prostaglandin F2 | a0.033 mg/ml |
| Erythromycin | 0.272 mg/ml | Prostaglandin E2 | 0.033 mg/ml |
| Fitsari na Uwar Uku na uku | 5% (volt) | MonistatR (miconazole) | 0.5 mg/ml |
| Oxytocin | 10 IU/ml | Indigo Carmine | 0.232 mg/ml |
| Terbutaline | 3.59 mg/ml | Gentamicin | 0.849 mg/ml |
| Dexamethasone | 2.50 mg/ml | Betadine Gel | 10 mg/ml |
| MgSO4•7H2O | 1.49 mg/ml | BetadineR Cleanser | 10 mg/ml |
| Ritodrine | 0.33 mg/ml | K-YR Jelly | 62.5 mg/ml |
| DermicidolR 2000 | 25.73 mg/ml |
MAGANAR LITTAFI
1. Kwalejin Kwararrun Ma'aikatan Lafiya ta Amurka.Ma'aikata na farko.Bulletin Fasaha, Lamba 133, Oktoba, 1989.
2. Creasy RK, Resnick R. Magungunan Mater da Fetal: Ka'idoji da Ayyuka.Philadelphia: WB Saunders;1989.
3. Creasy RK, Merkatz IR.Rigakafin haihuwa kafin haihuwa: ra'ayi na asibiti.Obstet Gynecol 1990;76 (Kashi 1): 2S–4S.
4. Morrison JC.Haihuwar da ba a kai ba: wasa mai wuyar warwarewa.Obstet Gynecol 1990;76 (Kashi 1): 5S-12S.
5. Lockwood CJ, Senyei AE, Dische MR, Casal DC, et al.Fibronectin tayi a cikin mahaifar mahaifa da ta farji a matsayin mai hasashen isar da haihuwa.Sabon Engl J Med 1991;325:669–74.
KALMOMIN ALAMOMIN
|
| Lambar kasida |  | Ƙayyadaddun yanayin zafi |
 | Tuntuɓi umarnin don amfani |
| Batch code |
 | Na'urar bincike ta in vitro |  | Yi amfani da ta |
 | Mai ƙira |  | Ya ƙunshi isa dongwaje-gwaje |
 | Kada a sake amfani |  | Wakili mai izini a cikin Ƙungiyar Turai |
 | CE mai alama bisa ga umarnin IVD Medical Devices 98/79/EC | ||
Abubuwan da aka bayar na Liming Bio-Products Co., Ltd.
No. 12 Huayuan Road, Nanjing, Jiangsu, 210042 PR China.
Tel: (0086)25 85476723 Fax: (0086)25 85476387
Imel:sales@limingbio.com
Yanar Gizo: www.limingbio.com
www.stdiagnostics.com
www.stidiagnostics.com
WellKang Ltd.(www.CE-marking.eu) Tel: +44(20)79934346
29 Harley St., London WIG 9QR, UK Fax: +44(20)76811874
StrongStep® Fetal Fibronectin Gwajin Saurin Na'urar
Bayarwa da wuri, wanda Cibiyar Nazarin Magunguna ta Amirka ta ayyana a matsayin bayarwa kafin mako na 37 na ciki, yana da alhakin yawancin cututtuka da mace-mace marasa chromosomal.Alamomin barazanar haihuwa kafin haihuwa sun hada da ciwon mahaifa, canjin zubar da jini, zubar jinin al'ada, ciwon baya, rashin jin dadi na ciki, matsawar pelvic, da kuma kumbura.Hanyoyin bincike don gano barazanar haihuwa kafin haihuwa sun haɗa da sa ido kan ayyukan mahaifa da aikin gwajin ƙwayar mahaifa na dijital, wanda ke ba da izinin kimanta girman mahaifa.
StrongStep® Fetal Fibronectin Gwajin Saurin Gwajin Gwajin Immunochromatographic na gani da aka fassara da nufin yin amfani da shi don gano ƙimar fibronectin tayi a cikin ɓoye na mahaifa tare da halaye masu zuwa:
Abokan mai amfani:hanya daya-mataki a gwajin inganci
Mai sauri:mintuna 10 kacal da ake buƙata yayin ziyarar mara lafiya ɗaya
Babu kayan aiki:asibitocin da ke iyakance tushen tushen ko saitin asibiti na iya yin wannan gwajin
Bayarwa:zafin jiki (2 ℃-30 ℃)
