Gwajin Antigen HSV 12
GABATARWA
HSV ambulaf ce, kwayar cuta mai ɗauke da DNA mai kama da ɗayanMambobin halittar Herpesviridae. Nau'o'in antigenically guda biyu negane, sanya nau'in 1 da nau'in 2.
Nau'in HSV na 1 da na 2 ana yawan kamuwa da su a cikin cututtukan da ba a sani ba na bakarami, fata, ido da al'aura, Cututtuka na tsakiyar juyayitsarin (meningoencephalitis) da kamuwa da cuta mai tsanani a cikin jaririAna kuma ganin majinyatan da ba su da rigakafi, ko da yake da wuya.Bayan dana farko kamuwa da cuta da aka warware, kwayar cutar na iya wanzu a boye a cikin mnama, daga inda zai iya sake fitowa, ƙarƙashin wasu sharuɗɗa, don haifar da amaimaita bayyanar cututtuka.
Maganganun asibiti na gargajiya na cututtukan al'aura yana farawa da tartsatsiMacules masu raɗaɗi da yawa da papules, waɗanda sannan suka girma cikin gungu na bayyane,vesicles masu cike da ruwa da pustules.Vesicles suna katsewa kuma suna haifar da ulcers.Fatar jikiulcers ɓawon burodi, alhãli kuwa raunuka a kan mucous membranes warke ba tare da crusting.A cikimata, ciwon ciki yana faruwa a cikin introitus, labia, perineum, ko perianal area.Mazayawanci suna tasowa raunuka a kan shingen azzakari ko glans.Mai haƙuri yakan tasowam inguinal adenopathy.Cututtukan mahaifa kuma suna da yawa a cikin MSM.Pharyngitis na iya tasowa tare da bayyanar baki.
Nazarin serology ya nuna cewa mutane miliyan 50 a Amurka suna da al'aurarHSV kamuwa da cuta.A Turai, ana samun HSV-2 a cikin 8-15% na yawan jama'a.A cikiAfirka, adadin yaɗuwar ya kai kashi 40-50% a cikin masu shekaru 20.HSV shine jagorasanadin ciwon al'aura.HSV-2 cututtuka aƙalla suna ninka haɗarin jima'isamun kwayar cutar rigakafi ta mutum (HIV) da kuma karuwawatsawa.
Har zuwa kwanan nan, warewar hoto ko bidiyo mai zagaya yanar gizo da sauri a al'adar tantanin halitta da ƙaddarar nau'in HSVtare da tabo mai kyalli ya kasance babban jigon gwajin herpes a cikin marasa lafiyagabatar da halayen halayen al'aura.Bayan gwajin PCR na HSV DNAan nuna shi ya fi kulawa fiye da al'adar hoto ko bidiyo mai zagaya yanar gizo da sauri kuma yana da takamaiman abin daya wuce 99.9%.Amma waɗannan hanyoyin a cikin aikin asibiti a halin yanzu suna iyakance,saboda farashin gwajin da ake buƙata don ƙwararrun, horarwama'aikatan fasaha don yin gwajin sun hana amfani da su.
Hakanan akwai samfuran gwajin jini na kasuwanci da ake amfani da su don gano nau'inTakamaiman ƙwayoyin rigakafi na HSV, amma waɗannan gwajin serological ba za su iya gano na farko bakamuwa da cuta don haka ana iya amfani da su kawai don kawar da cututtukan da ke faruwa.Wannan sabon gwajin antigen na iya bambanta sauran cututtukan ulcer da al'auraherpes, irin su syphilis da chancroid, don taimakawa farkon ganewar asali da maganiHSV kamuwa da cuta.
KA'IDA
An ƙera na'urar gwajin gaggawa ta HSV don gano antigen HSVta hanyar fassarar gani na ci gaban launi a cikin tsiri na ciki.TheAn cire membrane tare da anti-Herpes simplex virus monoclonal antibody a kan
yankin gwajin.Yayin gwajin, ana ba da izinin samfurin don amsawa tare da launimonoclonal anti-HSV antibody canza launin particals conjugates, wanda aka precoated a kansamfurin kushin gwajin.A cakuda sa'an nan motsa a kan membrane ta capillary
aiki, kuma yana hulɗa tare da reagents akan membrane.Idan akwai isassun HSVantigens a cikin samfurori, bandeji mai launi zai samar a yankin gwaji na membrane.Kasancewar wannan bandeji mai launi yana nuna sakamako mai kyau, yayin da rashi ya nuna
mummunan sakamako.Bayyanar band mai launi a yankin sarrafawa yana aiki azaman asarrafa tsari.Wannan yana nuna cewa an ƙara ƙimar samfurin daidaida kuma kumburi da membrane ya faru.