AMFANI DA NUFIN
Matakin Karfi^®Gwajin Procalcitonin shine saurin gwajin rigakafi-chromatographic don gano rabin ƙididdiga na Procalcitonin a cikin jini ko jini na ɗan adam.Ana amfani dashi don tantancewa da sarrafa magani mai tsanani, kamuwa da cuta na kwayan cuta da sepsis.

GABATARWA
Procalcitonin (PCT) ƙaramin furotin ne wanda ya ƙunshi ragowar amino acid 116 tare da nauyin kwayoyin halitta kusan 13 kDa wanda Moullec et al ya fara bayyana shi.a 1984. Ana samar da PCT kullum a cikin C-cell na glandar thyroid.A cikin 1993, an ba da rahoton girman matakin PCT a cikin marasa lafiya tare da tsarin kamuwa da cuta na asalin kwayan cuta kuma yanzu ana ɗaukar PCT a matsayin babban alamar cuta tare da kumburin tsarin da sepsis.Ƙimar tantancewar PCT tana da mahimmanci saboda kusancin kusanci tsakanin tattarawar PCT da tsananin kumburi.An nuna cewa "mai kumburi" ba a samar da PCT a cikin ƙwayoyin C.Kwayoyin asalin neuroendocrine sune mai yiwuwa tushen PCT yayin kumburi.

KA'IDA
Matakin Karfi^®Gwajin gaggawa na Procalcitonin yana gano Procalcitonin ta hanyar fassarar gani na ci gaban launi akan tsiri na ciki.Procalcitonin monoclonal antibody ba shi da motsi a yankin gwaji na membrane.Yayin gwaji, samfurin yana amsawa da ƙwayoyin rigakafin ƙwayoyin cuta na monoclonal anti-Procalcitonin waɗanda aka haɗa su zuwa barbashi masu launi kuma an riga an sanya su a kan kushin haɗin gwiwa na gwajin.Cakuda daga nan yana ƙaura ta cikin membrane ta aikin capillary kuma yana hulɗa tare da reagents akan membrane.Idan akwai isassun Procalcitonin a cikin samfurin, bandeji mai launi zai yi a yankin gwaji na membrane.Kasancewar wannan bandeji mai launi yana nuna sakamako mai kyau, yayin da rashi yana nuna mummunan sakamako.Bayyanar band mai launi a yankin sarrafawa yana aiki azaman tsarin kulawa, yana nuna cewa an ƙara ƙarar samfurin da ya dace kuma wicking membrane ya faru.Haɓaka launi daban-daban a cikin yankin layin gwaji (T) yana nuna sakamako mai kyau yayin da adadin Procalcitonin na iya ƙididdige shi da ƙima ta hanyar kwatanta ƙarfin layin gwajin zuwa ƙarfin layin tunani akan katin fassarar.Rashin layin launi a yankin layin gwaji(T)
yana nuna sakamako mara kyau.

MATAKAN KARIYA
Wannan kit ɗin don amfanin bincike ne kawai na IN VITRO.
∎ Wannan kayan aikin don ƙwararrun ƙwararru ne kawai.
n Karanta umarnin a hankali kafin yin gwajin.
∎ Wannan samfurin ba ya ƙunsar kowane kayan tushen ɗan adam.
∎ Kar a yi amfani da abun ciki na kit bayan ranar karewa.
∎ Karɓar duk samfuran a matsayin masu iya kamuwa da cuta.
∎ Bi daidaitaccen tsarin Lab da ka'idojin kare lafiyar halittu don sarrafawa da zubar da abubuwan da ke da lahani.Lokacin da aikin tantancewa ya cika, zubar da samfuran bayan an cire su ta atomatik a 121 ℃ na akalla 20 min.A madadin, ana iya bi da su tare da 0.5% Sodium Hypochlorite na awanni kafin a zubar.
∎ Kada a yi amfani da pipette ta baki kuma kada a shan taba ko ci yayin da ake tantancewa.
■ Sanya safar hannu yayin duk aikin.