Gwajin Saurin PROM
AMFANI DA NUFIN
Matakin Karfi®Gwajin PROM shine fassarar gani, ingantaccen gwajin immunochromatographic don gano IGFBP-1 daga ruwan amniotic a cikin ɓoyewar farji yayin daukar ciki.An yi nufin gwajin don yin amfani da ƙwararru don taimakawa wajen gano fashewar membranes na tayin (ROM) a cikin mata masu ciki.
GABATARWA
Matsakaicin IGFBP-1 (insulin-kamar abubuwan haɓakar haɓakar furotin-1) a cikin ruwan amniotic ya ninka sau 100 zuwa 1000 sama da na ƙwayar mahaifa.IGFBP-1 ba yawanci ba ne a cikin farji, amma bayan fashewar membranes na tayin, ruwan amniotic tare da babban taro na IGFBP-1 yana haɗuwa tare da ɓoyewar farji.A cikin gwajin StrongStep® PROM, ana ɗaukar samfurin ƙwayar ƙwayar cuta tare da swab polyester bakararre kuma ana fitar da samfurin a cikin Maganin Cire Samfura.Ana gano kasancewar IGFBP-1 a cikin maganin ta amfani da na'urar gwaji mai sauri.
KA'IDA
Matakin Karfi®Gwajin PROM yana amfani da immunochromatographic launi, fasahar kwararar capillary.A gwajin hanya na bukatar solubilization na IGFBP-1 daga farji swab ta hadawa da swab a Sample Buffer.Sa'an nan kuma za a ƙara gauraye samfurin buffer zuwa gwajin kaset da kyau kuma cakuda yana ƙaura tare da saman membrane.Idan IGFBP-1 ne ba a cikin samfurin, shi zai samar da wani hadaddun tare da primary anti-IGFBP-1 antibody conjugated zuwa canza launin barbashi.Sa'an nan za a daure hadaddun da wani na biyu anti-IGFBP-1 antibody mai rufi a kan nitrocellulose membrane.Bayyanar layin gwajin bayyane tare da layin sarrafawa zai nuna sakamako mai kyau.
KIT ABUBUWAN
| 20 Kowane mutum pakced gwajin na'urorin | Kowace na'ura tana ƙunshe da tsiri mai launin conjugates da reactive reagents waɗanda aka riga aka yi musu rufi a yankuna masu dacewa. |
| 2Ana cirewaBuffer vial | 0.1 M Phosphate buffered saline (PBS) da 0.02% sodium azide. |
| 1 swab mai inganci (kan buƙata kawai) | Ya ƙunshi IGFBP-1 da sodium azide.Don sarrafawa na waje. |
| 1 swab mara kyau (kan buƙata kawai) | Ba ya ƙunshi IGFBP-1.Don sarrafa waje. |
| 20 Bututun cirewa | Don samfuran shirye-shiryen amfani. |
| 1 Wurin aiki | Wuri don riƙe buffer vials da bututu. |
| 1 Saka kunshin | Don umarnin aiki. |
KAYAN DA AKE BUKATA AMMA BA'A SAMU BA
| Mai ƙidayar lokaci | Don amfani da lokaci. |
MATAKAN KARIYA
∎ Don ƙwararrun ƙwararrun bincike na in vitro amfani kawai.
■ Kada a yi amfani bayan ranar karewa da aka nuna akan kunshin.Kar a yi amfani da gwajin idan jakar jakar ta ta lalace.Kar a sake amfani da gwaje-gwaje.
Wannan kit ɗin ya ƙunshi samfuran asalin dabba.Ingantacciyar masaniyar asalin da/ko yanayin tsaftar dabbobi baya bada garantin gabaɗayan rashi ƙwayoyin cuta masu yaduwa.Don haka, ana ba da shawarar cewa a kula da waɗannan samfuran azaman masu iya kamuwa da cuta, kuma a sarrafa su tare da kiyaye ƙa'idodin aminci na yau da kullun (kada a sha ko shaƙa).
∎ Guje wa ƙetaren samfura ta hanyar amfani da sabon akwati na tattara samfuran ga kowane samfurin da aka samu.
■ Karanta tsarin duka a hankali kafin yin kowane gwaji.
■ Kada ku ci, sha ko shan taba a wurin da ake sarrafa samfurori da kayan aiki.Yi amfani da duk samfuran kamar suna ɗauke da ƙwayoyin cuta.Kula da ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙwayoyin cuta a duk tsawon aikin kuma bi ƙa'idodin ƙa'idodin don zubar da samfuran daidai.Sanya tufafi masu kariya kamar sutturar dakin gwaje-gwaje, safar hannu da za a iya zubarwa da kariyar ido lokacin da aka tantance samfurori.
∎ Kar a yi musanya ko hada reagents daga kuri'a daban-daban.Kar a haxa iyakoki na mafita.
Danshi da zafin jiki na iya yin illa ga sakamako.
∎ Lokacin da aikin tantancewa ya ƙare, zubar da swabs a hankali bayan an haɗa su ta atomatik a 121 ° C na akalla minti 20.A madadin, ana iya bi da su tare da 0.5% sodium hypochloride (ko bleach-rike gida) na awa ɗaya kafin zubar.Ya kamata a jefar da kayan gwajin da aka yi amfani da su daidai da ƙa'idodin gida, jihohi da/ko na tarayya.
∎ Kada a yi amfani da goga na cytology tare da masu ciki.
AJIYA DA KWANTA
∎ Yakamata a adana kit ɗin a 2-30°C har sai an buga kwanan watan ƙarewa akan jakar da aka rufe.
■ Dole ne gwajin ya kasance a cikin jakar da aka rufe har sai an yi amfani da shi.
■ Kar a daskare.
∎ Yakamata a kula don kare abubuwan da ke cikin wannan kit din daga kamuwa da cuta.Kar a yi amfani da shi idan akwai shaidar gurɓataccen ƙwayar cuta ko hazo.Cututtukan halittu na kayan aikin rarrabawa, kwantena ko reagents na iya haifar da sakamako na ƙarya.
TATTAUNAWA DA MISALIN
Yi amfani kawai da Dacron ko Rayon tipped swabs bakararre tare da robobi.Ana ba da shawarar yin amfani da swab ɗin da masana'anta ke bayarwa (Ba a ƙunshi swabs a cikin wannan kit ɗin ba, don bayanin oda, da fatan za a tuntuɓi masana'anta ko masu rarraba gida, lambar kasida ita ce 207000).Ba a inganta swabs daga wasu masu kaya ba.Swabs tare da tukwici na auduga ko katako na katako ba a ba da shawarar ba.
Ana samun samfurin ta amfani da swab polyester bakararre.Ya kamata a tattara samfurin kafin yin gwajin dijital da / ko duban dan tayi na transvaginal.Kula da kada ku taɓa wani abu tare da swab kafin ɗaukar samfurin.A hankali saka bakin swab a cikin farji zuwa farji na baya har sai juriya ta hadu.A madadin za'a iya ɗaukar samfurin daga fornix na baya yayin gwajin speculum mara kyau.Ya kamata a bar swab a cikin farji na tsawon daƙiƙa 10-15 don ba shi damar ɗaukar sigar farji.Cire swab ɗin a hankali!.
■ Sanya swab zuwa bututun cirewa, idan ana iya yin gwajin nan da nan.Idan gwajin nan da nan ba zai yiwu ba, ya kamata a sanya samfuran marasa lafiya a cikin busassun bututun sufuri don ajiya ko jigilar kaya.Ana iya adana swabs na awanni 24 a zazzabi na ɗaki (15-30°C) ko sati 1 a 4°C ko fiye da wata 6 a -20°C.Duk samfuran yakamata a bar su su kai zafin daki na 15-30 ° C kafin gwaji.
TSARI
Kawo gwaje-gwaje, samfurori, buffer da/ko sarrafawa zuwa zafin jiki (15-30°C) kafin amfani.
■ Sanya bututu mai tsafta a wurin da aka keɓe na wurin aiki.Ƙara 1ml na Mai Buffer Extraction zuwa bututun hakar.
■ Saka swab ɗin a cikin bututu.Haxa maganin da ƙarfi ta hanyar jujjuya swab da ƙarfi a gefen bututun na tsawon aƙalla sau goma (yayin nitse).Ana samun sakamako mafi kyau lokacin da samfurin ya haɗu da ƙarfi a cikin maganin.
■ Fitar da ruwa mai yawa kamar yadda zai yiwu daga swab ta hanyar danna gefen bututun cirewa mai sassauƙa yayin da ake cire swab.Aƙalla 1/2 na maganin buffer samfurin dole ne ya kasance a cikin bututu don isassun ƙaura na capillary ya faru.Saka hular kan bututun da aka fitar.
Yi watsi da swab a cikin kwandon shara mai haɗari mai haɗari.
∎ Samfuran da aka ciro na iya ajiyewa a cikin daki na tsawon mintuna 60 ba tare da sun shafi sakamakon gwajin ba.
∎ Cire gwajin daga jakar da aka hatimi, sa'annan a sanya shi a kan tsaftataccen wuri.Yi wa na'urar lakabi da majiyyaci ko ganewar sarrafawa.Don samun sakamako mafi kyau, yakamata a yi gwajin a cikin sa'a ɗaya.
∎ Ƙara ɗigo 3 (kimanin 100 µl) na samfurin da aka ciro daga Tube Extraction zuwa samfurin rijiyar akan kaset ɗin gwaji.
Guji kama kumfa mai iska a cikin samfurin rijiyar (S), kuma kar a jefa kowane bayani a cikin taga kallo.
Yayin da gwajin ya fara aiki, za ku ga launi yana motsawa a cikin membrane.
■ Jira band(s) masu launin su bayyana.Ya kamata a karanta sakamakon a minti 5.Kar a fassara sakamakon bayan mintuna 5.
Yi watsi da bututun gwajin da aka yi amfani da su da Kaset ɗin Gwaji a cikin kwandon shara mai dacewa.
FASSARAR SAKAMAKO
| KYAUTASAKAMAKO:
| Makada masu launi biyu suna bayyana akan membrane.Ƙungiya ɗaya yana bayyana a cikin yankin sarrafawa (C) kuma wani rukunin yana bayyana a yankin gwaji (T). |
| KARANTASAKAMAKO:
| Ƙungiya mai launi ɗaya kawai ya bayyana a cikin yankin sarrafawa (C).Babu makaɗa mai launi da ya bayyana a yankin gwaji (T). |
| BA YAWASAKAMAKO:
| Ƙungiyar sarrafawa ta kasa bayyana.Sakamako daga kowane gwajin da bai samar da rukunin sarrafawa ba a ƙayyadadden lokacin karantawa dole ne a watsar da shi.Da fatan za a sake duba tsarin kuma a maimaita tare da sabon gwaji.Idan matsalar ta ci gaba, daina amfani da kit ɗin nan da nan kuma tuntuɓi mai rarrabawa na gida. |
NOTE:
1. Ƙarfin launi a yankin gwaji (T) na iya bambanta dangane da ƙaddamar da abubuwan da ake nufi da ke cikin samfurin.Amma matakin abubuwan ba za a iya ƙayyade ta wannan gwajin ingancin ba.
2. Rashin isassun ƙarar samfurin, tsarin aiki da ba daidai ba, ko yin gwaje-gwajen da suka ƙare sune mafi kusantar dalilai na gazawar bandeji.
KYAUTATA KYAUTA
∎ An haɗa tsarin sarrafawa na ciki a cikin gwajin.Ƙungiya mai launi da ke bayyana a cikin yankin sarrafawa (C) ana ɗaukarsa azaman ingantaccen tsarin kulawa na ciki.Yana tabbatar da isassun ƙarar samfurin da ingantacciyar dabarar tsari.
∎ Ana iya samar da tsarin sarrafawa na waje (kan buƙata kawai) a cikin kayan don tabbatar da cewa gwaje-gwajen suna aiki yadda ya kamata.Hakanan, ana iya amfani da Sarrafa don nuna kyakkyawan aiki ta afaretan gwaji.Don yin gwajin sarrafawa mai inganci ko mara kyau, kammala matakan da ke cikin sashin Tsarin Gwaji da ke kula da swab mai sarrafawa daidai da swab samfuri.
IYAKA NA GWAJI
1. Bai kamata a yi tafsirin adadi bisa sakamakon gwajin ba.
2.Do not amfani da gwajin idan ta aluminum tsare jakar ko hatimin jakar ba m.
3.A tabbatacce Strong Mataki®Sakamakon gwajin PROM, kodayake gano kasancewar ruwan amniotic a cikin samfurin, bai gano wurin da ya fashe ba.
4.Kamar yadda tare da duk gwaje-gwajen bincike, dole ne a fassara sakamakon a cikin hasken sauran binciken asibiti.
5.Idan fashewar membranes na tayin ya faru amma zubar da ruwa na amniotic ya daina fiye da sa'o'i 12 kafin a dauki samfurin, IGFBP-1 na iya zama lalacewa ta hanyar proteases a cikin farji kuma gwajin na iya ba da sakamako mara kyau.
HALAYEN YI
Tebur: Ƙarfafa Mataki®Gwajin PROM vs. Wani Gwajin PROM
|
Hankalin Dangi: |
| Wani iri |
| ||
| + | - | Jimlar | |||
| Mataki mai ƙarfi®PROM Gwaji | + | 63 | 3 | 66 | |
| - | 2 | 138 | 140 | ||
|
| 65 | 141 | 206 | ||
Analytic hankali
Mafi ƙarancin gano adadin IGFBP-1 a cikin samfurin da aka fitar shine 12.5 μg/l.
Abubuwa Masu Tsangwama
Dole ne a kula don kada a gurɓata mai amfani ko ɓoyewar mahaifa tare da man shafawa, sabulu, magungunan kashe kwayoyin cuta, ko mayukan shafawa.Man shafawa ko man shafawa na iya tsoma baki cikin jiki tare da sha samfurin akan na'urar.Sabulu ko maganin kashe kwayoyin cuta na iya tsoma baki tare da maganin antibody-antigen.
An gwada abubuwan da za su iya shiga tsakani a ɗimbin yawa waɗanda za a iya samun su da kyau a cikin ɓoyewar mahaifa.Abubuwan da ke gaba ba su tsoma baki a cikin binciken ba lokacin da aka gwada su a matakan da aka nuna.
| Abu | Hankali | Abu | Hankali |
| Ampicillin | 1.47 mg/ml | Prostaglandin F2 | 0.033 mg/ml |
| Erythromycin | 0.272 mg/ml | Prostaglandin E2 | 0.033 mg/ml |
| Fitsari na Uwar Uku na uku | 5% (volt) | MonistatR (miconazole) | 0.5 mg/ml |
| Oxytocin | 10 IU/ml | Indigo Carmine | 0.232 mg/ml |
| Terbutaline | 3.59 mg/ml | Gentamicin | 0.849 mg/ml |
| Dexamethasone | 2.50 mg/ml | Betadine Gel | 10 mg/ml |
| MgSO4•7H2O | 1.49 mg/ml | BetadineR Cleanser | 10 mg/ml |
| Ritodrine | 0.33 mg/ml | K-YR Jelly | 62.5 mg/ml |
| DermicidolR 2000 | 25.73 mg/ml |
MAGANAR LITTAFI
Erdemoglu da Mungan T. Muhimmancin gano nau'in haɓakar insulin-kamar haɓakar haɓakar furotin-1 a cikin ɓoyewar mahaifa: kwatancen gwajin nitrazine da ƙimar ƙimar ruwa na amniotic.Acta Obstet Gynecol Scand (2004) 83: 622-626.
Kubota T da Takeuchi H. Kimanta nau'ikan nau'ikan haɓakar insulin-kamar haɓakar furotin-1 azaman kayan aikin bincike don fashewar membranes.J Obstet Gynecol Res (1998) 24:411-417.
Rutanen EM et al.Ƙimar gwajin tsiri mai sauri don abubuwan haɓakar haɓakar insulin-kamar furotin-1 a cikin gano fashewar membranes na tayin.Clin Chim Acta (1996) 253:91-101.
Rutanen EM, Pekonen F, Karkkainen T. Ma'auni na nau'in haɓakar insulin-kamar haɓakar furotin-1 a cikin ɓoyewar mahaifa / farji: kwatanta tare da ROM-duba Membrane Immunoassay a cikin ganewar asali na ruptured membranes tayi.Clin Chim Acta (1993) 214:73-81.
KALMOMIN ALAMOMIN
|
| Lambar kasida |  | Ƙayyadaddun yanayin zafi |
 | Tuntuɓi umarnin don amfani |
| Batch code |
 | Na'urar bincike ta in vitro |  | Yi amfani da ta |
 | Mai ƙira |  | Ya ƙunshi isa dongwaje-gwaje |
 | Kada a sake amfani |  | Wakili mai izini a cikin Ƙungiyar Turai |
 | CE mai alama bisa ga umarnin IVD Medical Devices 98/79/EC | ||
