Gwajin Right
Amfani da aka yi niyya
Da ƙarfi®Gwajin alkawarin da aka gani shine gwajin immunochratomatographic gwajin don gano Igfbp-1 daga ruwa mai ruwa a cikin farji a lokacin daukar ciki. An yi gwajin don amfani da ƙwararru don taimakawa wajen gano tsinkayen ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar cuta (ROM) cikin mata masu ciki.
Shigowa da
A maida hankali ne na IGFBP-1 (insulin-kamar ci gaban da aka ɗaure furotin-1) a cikin ruwa na amniotic shine 100 zuwa 1000 sau sama da na ɗan mace. IGFBP-1 ba yawanci ba ne a cikin farji, amma bayan katsewa daga cikin matattarar membranes, ruwa mai guba tare da babban taro na Igfbp-1 gauraye tare da farjin farji. A cikin gwajin almubazzaranci, ana ɗaukar samfurin ɓataccen mai ɓoye tare da polyester polyester Swab da ƙirar ƙirar ta hanyar maganin hakar. Kasancewar Igfbp-1 a cikin mafita ana gano ta amfani da na'urar gwajin sauri.
Ƙa'ida
Da ƙarfi®Gwajin gwajin yana amfani da immunochratomographic mai launi, fasaha ta prillary ta gudana. Hanyar gwajin tana buƙatar narkewa na Igfbp-1 daga farjin farji ta hanyar haɗa swab a cikin Buffer Buffer. Daga nan sai aka ƙara samfurin buffer a cikin Cassette samfurin samfurin da kyau kuma cakuda ya yi ƙaura tare da membrane farfajiya. Idan iGFBP-1 yana cikin samfurin, zai samar da wani hadaddun tare da farkon anti- 1 engiyod conjugated barbashi. Daga nan sai aka ɗaure ta na biyu-Igfbp-1 antaliby mai rufi akan membrane na nitrocellulose membrane. Bayyanar layin gwaji da ake iya gani tare da layin sarrafawa zai nuna sakamako mai kyau.
Kayan aikin Kit
| 20 Daban-daban pakked na'urorin gwaji | Kowace na'ura ta ƙunshi tsiri tare da launuka masu launin conjugate da kuma reagents reagents pre-mai rufi a cikin yankuna masu dacewa. |
| 2HakarBuffer vial | 0.1 m phosphate buffered saline (pbs) da 0.02% sodium azide. |
| 1 ingantaccen iko swab (akan bukatar kawai) | Dauke da Igfbp-1 da sodium raide. Don iko na waje. |
| 1 mara kyau sarrafawa (akan bukatar kawai) | Ba a ƙunshi IGFBP-1 ba. Don iko na waje. |
| 20 Hakar bututu | Don amfanin samfuran samfuri. |
| 1 Aiki | Wurin don rike da vials da shambura. |
| 1 Kunshin sakawa | Don umarnin aiki. |
Abubuwan da ake buƙata amma ba a bayar ba
| Mai ƙidali | Don amfani da lokaci. |
MATAKAN KARIYA
■ Don ƙwararru a cikin amfani da bincike na vitro kawai.
Kada kuyi amfani da bayan ranar karewa akan kunshin. Kada kuyi amfani da gwajin idan an lalata abin da ya faru. Kada ku sake yin gwaji.
Kit ɗin yana dauke da samfuran dabbobi. Tabbataccen ilimin asalin da / ko yanayin tsabta na dabbobi ba ya gaba ɗaya yana ba da tabbacin rashin isar da wakilai masu ba da izinin Pathogenic. Saboda haka, ana bada shawarar waɗannan samfuran kamar yadda yuwuwar kamuwa da cuta, kuma suna riƙe da kiyaye matakan tsaro na yau da kullun (ba sa ruwa).
Guji nau'ikan gurnani ta amfani da sabon akwati na tattarawa ga kowane samfurin da aka samu.
■ Karanta tsarin aikin a hankali kafin yin kowane gwaji.
Kada ku ci, sha ko hayaki a yankin da samfurori da kayan da aka sarrafa suna aiki. Rike duk samfurori kamar idan sun ƙunshi wakilai masu kamuwa. Lura kafa matakan da aka tanada game da haɗarin ƙwayoyin cuta a duk faɗin kuma bi daidaitattun hanyoyin don abubuwan zubar da samfuri. Saka rigakafin kariya kamar riguna na dakin gwaje-gwaje, safofin hannu da safofin hannu yayin da samfurori suke da mahimmanci.
Kada a musaya ko haɗuwa daga kuri'a daban-daban. Kar a hadu da kwalban kwalban kwalban.
Yanayin zafi da zazzabi zai iya shafar sakamako.
Lokacin da aka kammala aikin Assay, zubar da Swabi a cikin Autoclaving su a 121 ° C na mintuna 20. A madadin haka, za a iya bi da su da 0.5% sodium hypochlaide (ko kuma gidan riƙe Bleach) na sa'a daya kafin zubar. Ya kamata a jefar da kayan gwajin da aka yi amfani da shi daidai da na gida, jihar da / ko dokokin tarayya.
Kada kuyi amfani da cytology goge tare da marasa lafiya masu juna biyu.
Adana da kwanciyar hankali
A cikin kit ɗin ya kamata a adana a 2-30 ° C har zuwa ƙarshen ranar da aka buga akan aljihun da aka rufe.
Halin dole ne ya kasance cikin aljihun da aka rufe har sai amfani.
Kada a daskare.
■ Kiyaye ya kamata a ɗauka don kare abubuwan da aka gyara a cikin wannan kit ɗin daga gurbata. Kada kayi amfani idan akwai shaidar gurɓataccen ƙwayar cuta ko hazo. Dalilin halittar halittu ya gurɓatar kayan aiki, kwantena ko kuma reagents na iya haifar da sakamakon karya.
Samfuran samfuran da ajiya
Yi amfani da Dacron kawai ko Rayon Tip swabs tare da filastik filastik. Ana ba da shawarar yin amfani da Swabu da aka kawo ta da kayan ƙirƙirar (da swabs ba a ciki a cikin wannan kit ɗin, don ƙarin bayani, tuntuɓi mai kunnawa na gida, lambar Cataloge 207000). Swabs daga wasu masu samarwa ba a inganta su ba. Swabs tare da auduga na auduga ko ba a bada shawarar shings na katako ba.
Ana samun samfurin samfurin ta amfani da polyester bakararre swab. Ya kamata a tattara samfurin kafin yin jarrabawar dijital da / ko duban dan tayi. Ku kula kada ku taɓa wani abu tare da swab kafin shan samfurin. A hankali saka tip na swab a cikin farji zuwa ga Fornix Fornix har sai juriya aka sadu da juriya. A madadin haka ana iya ɗaukar samfurin daga ɓangaren lantarki yayin gwajin ƙirar bakararre. Yakamata a bar swab a cikin farjin na 10-15 seconds don ba da damar ɗaukar murhun farji. Ja swab fita a hankali!.
A sa swab zuwa bututun hakar, idan ana iya gudanar da gwajin nan da nan. Idan gwajin nan da nan ba zai yiwu ba, ya kamata a sanya samfurori masu haƙuri a cikin bututun jigilar kayayyaki don ajiya ko sufuri. Ana iya adana Swabbs na tsawon awanni 24 a zazzabi a ɗakin (15-30 ° C) ko mako 1 a 4 ° C ko fiye da wata 6 a -20 ° C. Duk samfuran ya kamata a ba da izinin isa ga zafin jiki na 15-30 ° C kafin gwaji.
Hanya
Ku zo da gwaje-gwaje, samfurori, mai ɗorewa da / ko sarrafawa zuwa zazzabi dakin (15-30 ° C) kafin amfani.
A sa wani bututun hakar mai tsabta a cikin yankin da aka tsara na aikin. Add 1ml na hakar mai kawowa zuwa bututun hakar.
■ Sanya samfuran samfuran shiga cikin bututun. Mixly Mix bayani ta hanyar juya swab da ƙarfi a kan gefen bututun na ƙalla sau goma (yayin baƙin ciki). Ana samun sakamako mafi kyau lokacin da samfurin yake gauraye a cikin mafita.
■ Jarra fitar da ruwa mai yawa kamar yadda zai yiwu daga swab ta pinching gefen hakar hakar mai sassauƙa yayin da aka cire swab. Aƙalla 1/2 na samfurin buffer bayani dole ya ci gaba da kasancewa a cikin bututu don isasshen ƙaurara don faruwa. Sanya hula a kan tube da aka fitar.
Jefar da swab a cikin akwati mai dacewa na liohajinous.
■ Samfuran da aka fitar dasu na iya riƙe su a zazzabi a daki tsawon minti 60 ba tare da shafar sakamakon gwajin ba.
Cire gwajin daga aljihun da aka rufe, kuma sanya shi a kan tsabta, matakin farfajiya. Yi lambar na'urar da mai haƙuri ko sarrafawa. Don samun sakamako mafi kyau, ya kamata a yi assay a cikin awa daya.
■ Gaba 3 saukad da (kamar 100 μL) na fitar da samfurin daga bututun mai zuwa samfurin da kyau a kaset gwajin.
Guji tafto kumfa iska a cikin samfuran da kyau (s), kuma kada ku cire wani bayani a cikin taga allon.
Kamar yadda gwajin ya fara aiki, za ka ga launi yana motsawa a saman membrane.
Dakata don buɗaɗɗen launuka na canza. Ya kamata a karanta sakamakon a minti 5. Kada ku fassara sakamakon bayan 5 da minti.
Jiran amfani da tubes gwaji da kuma kaset na gwaji a cikin akwati mai dacewa.
Neterpretation na sakamako
| MSakamako:
| Biyu masu launin launuka biyu suna bayyana akan membrane. Bandungiya ɗaya ta bayyana a yankin sarrafawa (c) da wani ƙungiyar ya bayyana a cikin yankin gwajin (t). |
| MSakamako:
| Bandaya daga cikin Band Band ya bayyana a yankin sarrafawa (c). Babu wani Band Band Band ya bayyana a cikin yankin gwajin (t). |
| Ba daidai baSakamako:
| Kulawa band sun kasa bayyana. Sakamako daga kowane gwaji wanda bai samar da ƙungiyar sarrafawa a lokacin karanta lokacin da dole ne a watsar. Da fatan za a duba hanya kuma maimaita tare da sabon gwaji. Idan matsalar ta ci gaba, dakatar da yin amfani da kit ɗin nan da nan kuma tuntuɓi mai raba gida. |
SAURARA:
1. Tsanani na launi a cikin yankin gwaji (t) na iya bambanta dangane da maida hankali kan abubuwa masu dacewa da aka gabatar a cikin samfuran. Amma ba za a iya tantance matakin abubuwa ba ta wannan gwajin cancantar.
2. Rashin ingantaccen girman samfurin, tsari ba daidai ba, ko yin gwaje-gwaje na ƙare shine mafi yawan dalilai na gazawar ƙungiya.
Iko mai inganci
Shirye-shiryen sarrafawa na ciki an haɗa su a cikin gwajin. Ana amfani da bandeji mai launin launi wanda ke bayyana a cikin yankin sarrafawa (C) a matsayin ikon aiwatar da ingantaccen tsari na ciki. Ya tabbatar da isasshen ƙimar ƙirar da madaidaiciyar hanyar aiwatarwa.
Ana iya samar da tsarin sarrafawa na waje na waje (akan buƙata) a cikin abubuwan don tabbatar da cewa gwaje-gwajen suna aiki yadda yakamata. Hakanan, za a iya amfani da sarrafawa don nuna yadda ya dace da aikin gwajin. Don yin tabbataccen sarrafawa ko mummunan aiki, kammala matakan a sashin tsarin gwajin kula da kula da swab a matsayin samfuran tantancewa.
Iyakokin gwajin
1. Ba za a yi fassarar da yawa ba bisa sakamakon gwajin.
2.Da yi amfani da gwajin idan kayan aikinsa na aluminium ko kuma hatimin jouch ba su da hankali.
3.a tabbatacce karfin gwiwa®Sakamakon gwajin, duk da cewa yana gano gaban ruwan amniotic ruwa a cikin samfurin, baya gano shafin na rupture.
4.as tare da duk gwaje-gwajen bincike, dole ne a fassara shi cikin hasken wasu binciken asibiti.
5.IF Rucupture na kariyar ƙwayar ƙwayar ƙwayar ƙwayar cuta ta faru amma lalataccen ruwa na amniotic ya daina sama da sa'o'i 12 kafin ƙayyadaddun abubuwan karewa da gwajin na iya ba da mummunan sakamako.
Halaye na aiki
Tebur: Mai ƙarfi®Alkawarin Gwada VS. Wani Alamar Alkawari
|
Dangi mai hankali: |
| Wani alama |
| ||
| + | - | Duka | |||
| Yar amarya®M Gwadawa | + | 63 | 3 | 66 | |
| - | 2 | 138 | 140 | ||
|
| 65 | 141 | 206 | ||
Analytic Sendurity
Mafi ƙarancin adadin adadin Igfbp-1 a cikin samfurin da aka fitar shine 12.5 μg / l.
Abubuwan shiga tsakani
Dole ne a kula da kulawa kar a gurbata korar mai nema ko cervicovaginal a cikin mai tare da madricts, sabulu, masu maganin maye, ko creams. Lickricants ko creams na iya tsoma baki tare da sha da ƙirar akan mai nema. Soaps ko masu shan hankali na iya tsoma baki tare da maganin antitod-antigen dauki.
An gwada abubuwa masu saurin shiga a maida hankali wanda za a iya samun mai da hankali a cikin ɓoye na mahaifa. Abubuwa masu zuwa ba su tsoma baki ba a cikin assay lokacin da aka gwada a matakin da aka nuna.
| Abu | Taro | Abu | Taro |
| Amicicillin | 1.47 MG / ML | Prostaglandin F2 | 0.033 MG / ML |
| Erythromycin | 0.272 MG / ML | Prostaglandin e2 | 0.033 MG / ML |
| Mahaifiyar Murfin 3rdter | 5% (vol) | Monistatr (micomezole) | 0.5 mg / ml |
| Omyttocin | 10 IU / ML | Indigo Carmine | 0.232 MG / ML |
| Terbutaline | 3.59 MG / ML | Ɗalibin | 0.849 MG / ML |
| Dexamehasone | 2.50 MG / ML | Betadener gel | 10 mg / ml |
| Mgso4•7H2O | 1.49 MG / ML | Betadener Tsabtace | 10 mg / ml |
| Riteodrine | 0.33 MG / ML | K-YR Jelly | 62.5 mg / ml |
| Dermicidolr 2000 | 25.73 MG / ML |
Tunani
Erdemoglu da Mungan T. Muhimmancin gano asalin insulin-1 a cikin cervicaral securations - 1 Cervicovaginal Sorrations - 1 Cervicovagalin gwajin nitrazine da ƙididdigar Nitrazine. Actta obstet Gyneecol Scand (2004) 83: 622-626.
Kubota T da Takami H. Extarationiationididdigar ci gaban insulin-kamar ci gaban ci gaban furotin-1 a matsayin kayan aikin bincike don katsewa daga membranes. J obstet Gynecol Res (1998) 24: 411-417.
Rutaneen em et al. Kimulawar gwajin tsibi na gwaji na kamarular-kamar ci gaban ci gaban furotin-1 a cikin ganewar asali na membranes na retal membranes. CLIM ACTA (1996) 253: 91-101.
Rutanenen EM, Pokonen F, Karkkarn da insulin-kamar ci gaban ƙasa mai girma: kwatankwacin ɓoye na membranes CLIM ACTA (1993) 214: 73-81.
Mai haske na alamomi
|
| Lambar Katalog |  | Tsarin zafin jiki |
 | Taimaka wa umarnin don amfani |
| Lambar tsari |
 | A cikin na'urar kiwon lafiya na Vitro |  | Amfani da |
 | Mai masana'anta |  | Ya isa ya isa |
 | Kada ku sake amfani |  | Wakilin izini a cikin jama'ar Turai |
 | Ce a cewar na'urori na IVD na IVD 98/79 / EC | ||
