Gwajin gaggawa na Rotavirus Antigen
GABATARWA
Rotavirus shine mafi yawan wakili da ke da alhakin gastroenteritis mai tsanani, yawanci a cikin yara ƙanana.Gano shi a cikin 1973 da haɗin gwiwa tare da jarirai gastro-enteritis suna wakiltar ci gaba mai mahimmanci a cikin nazarin gastroenteritis wanda ba ya haifar da kamuwa da cuta mai tsanani.Ana kamuwa da cutar ta Rotavirus ta hanyar baka-baki tare da lokacin shiryawa na kwanaki 1-3.Kodayake samfuran da aka tattara a cikin rana ta biyu da ta biyar na rashin lafiya sun dace don gano antigen, ana iya samun rotavirus yayin da zawo ke ci gaba.Rotaviral gastroenteritis na iya haifar da mace-mace ga mutanen da ke cikin haɗari kamar jarirai, tsofaffi da marasa lafiya na rigakafi.A cikin yanayin zafi, cututtukan rotavirus suna faruwa ne musamman a cikin watanni na hunturu.An ba da rahoton bullar cutar da kuma annoba da ta shafi wasu dubunnan mutane.Tare da yaran da aka kwantar da su a asibiti suna fama da matsanancin ciwon ciki, har zuwa kashi 50% na samfuran da aka bincika sun tabbata ga rotavirus.Kwayoyin cuta suna yin kwafi a cikin
Kwayoyin tantanin halitta da tend tend to don karbar bakuncin jinsin-takamaiman samar da halayyar cututtukan Cytopathatic (cpe).Saboda rotavirus yana da matuƙar wahala ga al'ada, baƙon abu ne a yi amfani da keɓewar kwayar cutar wajen gano cututtuka.Maimakon haka, an samar da dabaru iri-iri don gano rotavirus a cikin najasa.
KA'IDA
Na'urar Gwajin Saurin Rotavirus (Feces) tana gano rotavirus ta hanyar fassarar gani na ci gaban launi akan tsiri na ciki.Kwayoyin rigakafin rotavirus ba su da motsi a yankin gwaji na membrane.A lokacin gwaji, samfurin
yana amsawa da ƙwayoyin rigakafin rotavirus waɗanda aka haɗa su zuwa barbashi masu launi kuma an riga an riga an riga an rufe su a jikin samfurin gwajin.Cakuda daga nan yana ƙaura ta cikin membrane ta aikin capillary kuma yana hulɗa tare da reagents akan membrane.Idan akwai
isassun rotavirus a cikin samfurin, bandeji mai launi zai samar a yankin gwaji na membrane.Kasancewar wannan bandeji mai launi yana nuna sakamako mai kyau, yayin da rashi yana nuna mummunan sakamako.Bayyanar band mai launi a
yankin sarrafawa yana aiki azaman kulawar tsari, yana nuna cewa an ƙara ƙarar samfurin da ya dace kuma wicking membrane ya faru.
KIT ABUBUWAN
| Na'urorin gwaji da aka cika daban-daban | Kowace na'ura tana ƙunshe da tsiri mai launin conjugates da reactive reagents waɗanda aka riga aka yi musu rufi a yankuna masu dacewa. |
| Samfuran dilution bututu tare da buffer | 0.1 M Phosphate buffered saline (PBS) da 0.02% sodium azide. |
| pipettes masu zubarwa | Don tattara samfuran ruwa |
| Saka kunshin | Don umarnin aiki |
KAYAN DA AKE BUKATA AMMA BA'A SAMU BA
| Mai ƙidayar lokaci | Don amfani da lokaci |
| Centrifuge | Don maganin samfurori a cikin yanayi na musamman |
Takaddun shaida
