SARS-COV-2 Antigen Rapp (Amfani da Kwararru)
Amfani da aka yi niyya
Standarda-2 SARS-2 SARS - 2 Farkon ranar farko ta farkon bayyanar cututtuka. Ana amfani da assay azaman taimako a cikin ganewar asali-19. An yi amfani da tsarin zane don allon kamuwa da cuta da kuma bayyanar cututtukan auxilis da mutane masu asuwa da kuma asyptomatic mutane.
Shigowa da
Maganar Coronaviruse na β. COVID-19 cuta ce mai saurin cutar. Mutane gaba daya mai saukin kamuwa. A halin yanzu, marasa lafiya da cutar Coronavirus shine babbar hanyar kamuwa da cuta; Mutane masu kamuwa da cutar asympmmatomatic na iya zama tushen m. Dangane da bincike na yanzu na yanzu, lokacin shiryawa shine kwanaki 1 zuwa 14, mafi yawa 3 zuwa 7 kwanaki. Babban bayyanar sun hada da zazzabi, gajiya da bushe tari. Rashin ambaliya, hanci mai gudu, ciwon makogwaro, myalgia da gudawa ana samun 'yan karar lokuta.
Ƙa'ida
Gwajin Cokali na SARS COV-2 COV-2 gwajin Antigen ya ɗauki gwajin immunochracomatographic. LateX conjugated maganin rigakafi (L. Lawex-ab) ya dace da SARS-COV-2 sune bushe-impestbilized a ƙarshen Nitrocellulose Strip. SARS-2-2 Antibids na 2 suna da haɗin gwiwa a yankin gwajin (T) da biotin-BSA na haɗin kai ne (c). Idan ya kasance a cikin samfurin, SARS-Cov-2 antigens zai yi laushi tare da maganin maganin rigakafi da ke haifar da barbashi. Wadannan lamuran zasu ci gaba da yin ƙaura tare da tsiri har sai yankin gwajin (T) inda SARS-2 ya kama su. Idan babu SARS-2 antigens a cikin samfurin, babu layin ja layi a yankin gwajin (t). Za a ci gaba da yin hijira Kadai har sai an kama shi a yankin sarrafawa (c) da tarawar biotin-basa a cikin layin shuɗi, wanda ke nuna ingancin gwajin.
Kayan aikin Kit
| Poot na cokali 25 wanda aka rufe | Kowane na'urar ya ƙunshi tsiri tare da launuka masu launin conjugates da kuma sake dawo da karatuttukan da aka riga aka riga aka ba da su a daidai yankuna. |
| 25 hakar bututu tare da pre-cike dilutie dilution | 0.1 m phosphate buffered saline (pbs) da 0.02% sodiumzade. |
| 25 fakitoci na swab | Don samfuran samfuran samfuri. |
| 1 aiki | Wurin don rike da vials da shambura. |
| 1 Saka Saka | Don umarnin aiki. |
Kayan aikin Kit
| Mai ƙidali | Don amfani da lokaci. |
| Duk wani kayan kariya na yau da kullun |
MATAKAN KARIYA
• Wannan kit ɗin yana cikin amfani da bincike kawai.
• Wannan kit ɗin yana don amfanin ƙwarewar likita kawai.
• Karanta umarnin a hankali kafin yin gwajin.
• Wannan samfurin bai ƙunshi kowane kayan asalin ɗan adam ba.
• Kada kayi amfani da abubuwan da ke ciki bayan ranar karewa.
• rike duk samfurori a matsayin mai yiwuwa.
• Bi hanya madaidaiciya da kuma jagororin biosafy jagororin aiki da kuma zubar da kayan abinci mai iya lalacewa. Lokacin da aka kammala aikin assay, sai a yanka samfurori bayan sarrafa su a 121 ℃ na aƙalla minti 20. A madadin haka, ana iya bi da su tare da 0.5% sodium hypochlollorite hudu kafin zubar.
• Kada kayi maganin bututun ciki da baki kuma babu shan taba ko cin abinci yayin aiwatar da magoya.
• sanya safofin hannu a lokacin duka hanya.
• Ana ba da shawarar yin amfani da na'urar tsarin halittar bio don ganowa mai sauri na SARS-2 (cat # 500210) don kare ma'aikaci da yanayin.
Adana da kwanciyar hankali
Shafin da aka rufe a cikin kayan gwajin na iya adana tsakanin 2-30 ℃ don tsawon lokacin shiryayye kamar yadda aka nuna a kan jakar.
Samfuran samfuran da ajiya
Nasal swab samfurin:
• Sanya Swab guda ɗaya zuwa hancin mara lafiya ɗaya. Ya kamata a saka Tukar Swabi har zuwa 2.5 cm (1 inch) daga gefen hanci. Mirgine swab sau 5 tare da mucosa a cikin hancin hanci don tabbatar da cewa an tattara abubuwan gamsai.
• Yi amfani da swab iri ɗaya, maimaita wannan tsari na sauran hanci don tabbatar da cewa an tattara samfurin samfurin daga hanci da hanci.
Yi amfani da Swabu wanda aka kawo a cikin kit, Alwashin Swabs na iya shafar aiwatar da aikin gwaji, yana inganta ya inganta swab kafin amfani da shi. An ba da shawarar cewa za a sarrafa samfuran da wuri-wuri bayan tarin. Ana iya ɗaukar samfurori a cikin akwati har zuwa awa 1 a ɗakin zafin jiki (15 ° C zuwa 30 ° C), ko har zuwa awanni 24 lokacin da firiji (2 ° C) kafin aiki.
Hanya
Kawo na'urori na gwaji, samfurori, buffer da / ko sarrafawa zuwa zazzabi dakin (15-30 ° C) kafin amfani.
• Umart mai cike da mai cika fuska, cire hatimin daga vial dauke da liqutd.
• sanya ƙirar swab cikin bututu. Mixly Mix bayani ta jujjuya swab da ƙarfi a kan gefen bututun na ɗan sau 15 (yayin da aka nutsar). Ana samun sakamako mafi kyau lokacin da samfurin yake gauraye a cikin mafita.
• Bada izinin swab don jiƙa a cikin hakar wasan na minti daya kafin mataki na gaba
• Juya a cikin ruwa mai yawa kamar yadda zai yiwu daga swab ta zubar da gefen hakar mai sassauƙa yayin da aka cire swab. Aƙalla 1/2 na samfurin buffer bayani dole ya ci gaba da kasancewa a cikin bututu don isasshen ƙaurara don faruwa. Sanya hula a kan tube da aka fitar.
• Jefa da swab a cikin akwati mai dacewa na liohaardus.
• Rufe hula.
• Haɗa maganin ta hanyar matsi samfurin da ƙarfi da ƙarfi a gefen bututun na akalla sau goma
(yayin da aka mamaye). Ana samun sakamako mafi kyau lokacin da aka gauraye samfurin a cikin mafita. Bada izinin samfuri don jiƙa a cikin Buffer mai diloli na minti daya kafin mataki na gaba.
• Abubuwan da aka fitar dasu na iya riƙe su a zazzabi a daki tsawon minti 30 ba tare da shafar sakamakon gwajin ba.
Cire na'urar gwajin daga aljihun da aka rufe, kuma sanya shi a kan tsabta, matakin farfajiya. Yi lambar na'urar da mai haƙuri ko sarrafawa. Don samun sakamako mafi kyau, ya kamata a yi assay a cikin minti 30.
Addara 3 saukad da (kamar 100 μL) na fitar da samfurin daga bututun mai zuwa zagaye samfurin da kyau a kan na'urar gwajin.
• Guji yin tarko da kumfa a cikin samfurin da kyau (s), kuma kar a zubar da kowane bayani a cikin taga allon. Kamar yadda gwajin ya fara aiki, za ka ga launi yana motsawa a saman membrane.
• Jira Band Band (s) ɗin da aka canza launi ya bayyana. Ya kamata a karanta sakamakon ta hanyar gani a minti 15. Kada a fassara sakamakon bayan minti 30.
Jiran amfani da bututu na girke-girke da na'urorin gwaji a cikin kwandon shara mai dacewa.
Rubuta sakon ka a nan ka aika da shi
