Novel Coronavirus (SARS-CoV-2) Kit ɗin PCR na Real-Time Real-Time
Wannan kayan aikin PCR mai mahimmanci, shirye-shiryen amfani yana samuwa a cikin tsarin lyophilized (tsarin bushewa daskarewa) don adana dogon lokaci.Ana iya jigilar kayan kuma a adana shi a zazzabi na ɗaki kuma ya tsaya tsayin daka har tsawon shekara guda.Kowane bututu na premix ya ƙunshi duk na'urorin da ake buƙata don haɓakawa na PCR, gami da Reverse-transcriptase, Taq polymerase, primers, probes, da kuma dNTPs substrates.Yana buƙatar ƙara 13ul distilled ruwa da 5ul fitar da samfurin RNA, sannan ana iya gudu da haɓaka akan kayan PCR.
Na'urar qPCR yakamata ta cika buƙatun masu zuwa:
1. Fit 8 tsiri PCR tube girma 0.2 ml
2. Suna da tashoshin gano sama da huɗu:
| Tashoshi | Tashin hankali (nm) | Fitowa (nm) | Rini da aka riga aka yi calibrated |
| 1. | 470 | 525 | FAM, SYBR Green I |
| 2 | 523 | 564 | VIC, HEX, TET, JOE |
| 3. | 571 | 621 | ROX, TEXAS-JA |
| 4 | 630 | 670 | CY5 |
PCR- dandamali:
7500Real-Time PCR System, Biorad CF96, iCycler iQ™ Tsarin Gano PCR na Gaskiya, Stratagene Mx3000P, Mx3005P
Wahalar safarar sarkar sanyi na Novel Coronavirus nucleic acid reagent ganowa
Lokacin da na al'ada nucleic acid gano reagents ana hawa a cikin dogon nesa, da (-20± 5) ℃ sanyi sarkar ajiya da kuma harkokin sufuri ake bukata don tabbatar da bioactive na enzyme a cikin reagents ci gaba da aiki.Don tabbatar da cewa zafin jiki ya kai ga ma'auni, ana buƙatar kilogiram da yawa na busassun ƙanƙara don kowane akwati na reagent na gwajin nucleic acid ko da ƙasa da 50g, amma zai iya wucewa na kwanaki biyu ko uku kawai.Dangane da aikin masana'antu, ainihin nauyin reagents da masana'antun ke bayarwa bai wuce 10% (ko ƙasa da wannan ƙimar) na akwati ba.Yawancin nauyin ya fito ne daga busassun kankara, fakitin kankara da akwatunan kumfa, don haka farashin sufuri yana da yawa.
A cikin Maris 2020, COVID-19 ya fara bazuwa a cikin babban sikeli a ƙasashen waje, kuma buƙatun novel Coronavirus gano nucleic acid reagent ya karu sosai.Duk da babban farashin fitarwa da reagents a cikin sanyi sarkar, mafi masana'antun iya har yanzu yarda da shi saboda da babban yawa da babban riba.
Koyaya, tare da haɓaka manufofin fitarwa na ƙasa don samfuran rigakafin cutar, gami da haɓaka ikon ƙasa kan kwararar mutane da dabaru, akwai tsawaitawa da rashin tabbas a lokacin jigilar kayayyaki na reagents, wanda ya haifar da manyan matsalolin samfuran da suka haifar. ta hanyar sufuri.Tsawaita lokacin sufuri (lokacin jigilar kayayyaki na kusan rabin wata ya zama gama gari) yana haifar da gazawar samfur akai-akai lokacin da samfurin ya isa ga abokin ciniki.Wannan ya dami mafi yawan masana'antun nucleic acid reagents na fitar da kayayyaki.
Fasahar Lyophilized don PCR reagent ta taimaka jigilar Novel Coronavirus nucleic acid gano reagent a duk duniya
Za'a iya ɗaukar reagents na PCR na lyophilized da adana su a cikin zafin jiki, wanda ba zai iya rage farashin sufuri kawai ba, har ma da guje wa matsalolin ingancin da tsarin sufuri ya haifar.Saboda haka, lyophilizing da reagent ita ce hanya mafi kyau don magance matsalar sufurin fitarwa.
Lyophilization ya ƙunshi daskarewa bayani a cikin wani m yanayi, sa'an nan kuma sublimate da kuma raba ruwa tururi a karkashin injin yanayi.Busassun solute ya kasance a cikin akwati tare da abun da ke ciki da aiki iri ɗaya.Idan aka kwatanta da reagents na ruwa na al'ada, cikakken nau'in lyophilized Novel Coronavirus nucleic acid reagent wanda Liming Bio ya samar yana da halaye masu zuwa:
Matsakaicin kwanciyar hankali mai ƙarfi:zai iya tare da tsayawa magani a 56 ℃ for 60 days, da kuma ilimin halittar jiki da kuma yi na reagent kasance ba canzawa.
Ma'ajiya da sufuri na al'ada:babu buƙatar sarkar sanyi, babu buƙatar adanawa a ƙananan zafin jiki kafin buɗewa, cikakken sakin sararin ajiyar sanyi.
Shirye-shirye don amfani:lyophilizing na duk sassan, babu buƙatar tsarin tsarin, guje wa asarar abubuwan da aka gyara tare da babban danko kamar enzyme.
Multiplex hari a cikin bututu daya:Maƙasudin ganowa ya ƙunshi sabon coronavirus ORF1ab gene, N gene, S gene don guje wa ƙwayar cuta.Don rage rashin kuskuren ƙarya, ana amfani da kwayar halittar ɗan adam RNase P azaman kulawa ta ciki, don saduwa da buƙatun asibiti don sarrafa ingancin samfurin.
