Morelonavirus (SARS-COV-2) Kit ɗin Ka'idodi na yau da kullun
Wannan mai matukar hankali, shirye-don amfani da Kit ɗin PCR yana samuwa a cikin tsarin lyophilized (daskararre-bushewa) don ajiya na dogon lokaci. Za a iya jigilar kayan kuma a adana shi a zazzabi a daki kuma an bartar tsawon shekara guda. Kowace bututu na premix ya ƙunshi dukkanin reagents da ake buƙata don samfurin PCR amplification, gami da juzu'i, polymase, firikwenar, bincike, da dntps substrates. Yana buƙatar ƙara ruwa mai narkewa 13ul da 5ul an haɗa shi da izini na RNA, to, ana iya gudana da haɓaka kayan kida na PCR.
Injin qpcr yakamata ya cika wadannan bukatun:
1
2. Ka sami tashoshi sama da hudu:
| Hanya | SAURARA (NM) | Rage (nm) | Pre-Talibred Dyes |
| 1. | 470 | 525 | Fam, Sybr Green Ni |
| 2 | 523 | 564 | Vic, Hex, Tet, Joe |
| 3. | 571 | 621 | Rox, Texas-Red |
| 4 | 630 | 670 | Cy5 |
PCR-dandamali:
Tsarin PCR na 7500reAl-lokaci, biorad cf96, Icycler iq ™ tsarin ganowa, Stratagene Mx3000, MX30000
Da wahala na sarkar sarkar sarkar kayan aikin coronavirus na ganowa
Lokacin da gano abubuwan gano makaman acid na al'ada a nesa, da (-20 ± sarkar da aka yi da adana enzyme a cikin reagents ya kasance mai aiki. Don tabbatar da cewa yawan zafin jiki ya kai matsayin, kilo kilograms na bushewar kankara ana buƙatar kowane kwalin gwajin nucleic ko da ƙasa da kwana biyu ko uku kawai. A kan hangen masana'antu, ainihin nauyin reagents ya bayar ƙasa da 10% (ko kuma nesa da wannan darajar) na kwandon. Yawancin nauyi ya fito ne daga bushe kankara, kankara kankara da kwalaye na kumfa, don haka farashin sufuri yana da matuƙar girma.
A cikin Maris 2020, COVID-19 ya fara fashewa a cikin babban sikeli a kasashen waje, da kuma bukatar Motowa Coronavirus gano karagar makaman acid ya karu sosai. Duk da babban farashi na fitarwa da reagents a cikin sarkar sanyi, yawancin masana'antun za su iya yarda da shi saboda yawan ribar babba da yawa.
Koyaya, tare da inganta manufofin fitarwa na ƙasa don samfurori na anti-pungich, da kuma haɓaka iko na ƙasa a cikin lokacin jigilar kayayyaki, wanda ya haifar da manyan matsalolin da suka haifar ta hanyar sufuri. Lokacin sufuri (lokacin sufuri na kusan rabin wata daya ya zama ruwan dare na gama gari) yana haifar da gazawar samfurin sauƙaƙe lokacin da samfurin ya kai abokin ciniki ya kai abokin ciniki. Wannan ya wahalar da yawancin masana'antar fitarwa masana'antar fitarwa.
Ingantaccen fasaha na PCR Reagent ya taimaka wa jigilar kayayyaki na allo na Coronavirus gano mai sake dawo da shi na gaba
The lyophilized PCR reagents can be transported and stored at room temperature, which can not only reduce the transportation cost, but also avoid the quality problems caused by transportation process. Saboda haka, lyophilized reagent shine hanya mafi kyau don magance matsalar jigilar sufuri.
Lyophilization ya shafi daskarewa mafita a cikin m jihar, sannan kuma ya raba kuma raba tururin ruwa a ƙarƙashin yanayin iska. Duye da bushe ya kasance a cikin akwati tare da wannan abun da aiki. Idan aka kwatanta da real ruwa reagents, cikakken-kayan haɗin linafa Lyophilized Proverv acid Coronavirus gano da iyayen Bio yana da halaye masu zuwa:
Matuƙar ƙarfi mai ƙarfi na zafi:Zai iya tare da tsayayyen magani a 56 ℃ na kwanaki 60, da ilimin halittar jiki da kuma aikin da aka sake fasalin ba su canzawa ba.
Active zafin jiki na yau da kullun da sufuri:Babu buƙatar sarkar sanyi, babu buƙatar adanawa a cikin ƙarancin zafin jiki kafin a ba da labari, cikakken saki sarari ajiya mai sanyi.
Shirya-da-amfani:Lyophilized dukkan abubuwanda aka gyara, babu buƙatar daidaitawar tsarin, guje wa asarar abubuwanda ke da babban danko kamar enzyme.
Rukunin mahara a cikin bututu ɗaya:Gano ganowa ya mamaye gonar coronvirus orf1Ab Gene, nen gene don gujewa kwayar cutar. Don rage karya mara kyau, ana amfani da gene p garken na ciki azaman ikon ciki, don saduwa da buƙatar asibiti don ingancin ingancin samfurin.
