AMFANI DA NUFIN
Matakin Karfi^®HPV 16/18 Antigen Rapid Na'urar Gwajin Saurin Saurin Immunoassay ne na gani na gani don gano ƙimar ƙimar HPV 16/18 E6&E7 oncoproteins a cikin samfuran swab na mahaifa mata.An yi nufin yin amfani da wannan kit ɗin a matsayin taimako a cikin ganewar cutar sankarar mahaifa da ciwon daji.

GABATARWA
A kasashe masu tasowa, cutar sankarar mahaifa ita ce kan gaba wajen haddasa mutuwar mata masu alaka da cutar kansa, saboda rashin aiwatar da gwaje-gwajen gwajin cutar kansa kafin mahaifar mahaifa da kuma ciwon daji.Gwajin gwaji don ƙananan saitunan albarkatu yakamata ya zama mai sauƙi, mai sauri, da tasiri mai tsada.Da kyau, irin wannan gwajin zai zama mai ba da labari game da ayyukan oncogenic na HPV.Bayyana duka HPV E6 da E7 oncoproteins suna da mahimmanci don canjin cell na mahaifa ya faru.Wasu sakamakon bincike sun nuna alaƙar E6 & E7 oncoprotein positivity tare da tsananin tsananin histopathology na mahaifa da haɗarin ci gaba.Don haka, E6&E7 oncoprotein yayi alƙawarin zama maƙasudin halitta mai dacewa na ayyukan oncogenic na HPV.

KA'IDA
Matakin Karfi^®HPV 16/18 Antigen Rapid Na'urar An ƙera shi don gano HPV 16/18 E6&E7 Oncoproteins ta hanyar fassarar gani na ci gaban launi a cikin tsiri na ciki.An yi watsi da membrane tare da anti-HPV 16/18 E6&E7 rigakafi na monoclonal akan yankin gwajin.A lokacin gwajin, ana ba da izinin samfurin don amsawa tare da nau'ikan anti-HPV 16/18 E6&E7 masu launin ƙwayoyin cuta masu launi, waɗanda aka riga aka riga aka yi su akan kushin samfurin gwajin.Cakuda daga nan yana motsawa akan membrane ta aikin capillary, kuma yayi hulɗa tare da reagents akan membrane.Idan akwai isassun oncoproteins na HPV 16/18 E6&E7 a cikin samfurori, bandeji mai launi zai samar a yankin gwaji na membrane.Kasancewar wannan bandeji mai launin yana nuna sakamako mai kyau, yayin da rashinsa yana nuna mummunan sakamako.Bayyanar band mai launi a yankin sarrafawa yana aiki azaman kulawar tsari.Wannan yana nuna cewa an ƙara ƙarar samfurin da ya dace kuma an sami wicking membrane.

TATTAUNAWA DA MISALIN
■ Ingancin samfurin da aka samu yana da matuƙar mahimmanci.Kamar yaddaYa kamata a tattara tantanin epithelial na mahaifa ta swab.Don samfuran mahaifa:
∎ Yi amfani da swabs na Dacron ko Rayon kawai tare da robobi.Yana daba da shawarar yin amfani da swab ɗin da masana'anta ke bayarwa (Swab ɗin suneba a cikin wannan kit ɗin, don bayanin oda, da fatan za a tuntuɓimasana'anta ko masu rarraba gida, lambar kataloji shine 207000).Swabsdaga sauran masu samar da kayayyaki ba a tabbatar da su ba.Swabs tare da tukwici auduga koBa a ba da shawarar katako na katako ba.
∎ Kafin tarin samfuri, cire ƙoƙon ƙura daga yankin endocervicaltare da swab daban ko auduga a jefar.Saka swab a cikincervix har sai kawai mafi ƙarancin zaruruwa sun fallasa.Juya swab da ƙarfina 15-20 seconds a daya hanya.Cire swab a hankali!
∎ Kar a sanya swab a cikin kowace na'ura mai dauke da matsakaici tun lokacinsufuri matsakaici tsoma baki tare da kima da kuma yiwuwa na kwayoyin neba a buƙata don tantancewa.Sanya swab zuwa bututun cirewa, idan an gwadaza a iya gudu nan da nan.Idan gwajin gaggawa ba zai yiwu ba, mai haƙurisamfurori ya kamata a sanya su a cikin busassun bututun sufuri don ajiya ko jigilar kaya.TheAna iya adana swabs na sa'o'i 24 a zazzabi na ɗaki (15-30 ° C) ko mako 1a 4 ° C ko fiye da watanni 6 a -20 ° C.Ya kamata a ba da izinin duk samfurandon isa dakin zafin jiki na 15-30 ° C kafin gwaji.